目的 通过建立大鼠脑出血(intracerebral hemorrhage,ICH)模型,研究PPARγ激动剂(罗格列酮)对雄性SD大鼠脑出血后血肿周围小胶质细胞(M1、M2)极化的影响.方法 将48只雄性SD大鼠按照随机数字表法分为正常组(Control)、脑出血模型组(ICH)、氯化钠干预组(NaCl)、罗格列酮干预组(RSG),每组12只.用大鼠自体血建立ICH模型;各组大鼠分别于6、24、48、72 h时间点进行神经功能评分(Longa评分),采用免疫组化和光密度法检测血肿周围组织CD16、CD206表达情况.结果 各时间点ICH组的Longa评分均高于NaCl组(P＜0.05).各时间点RSG组的Longa评分均低于ICH组(P＜0.05).脑出血造模后6 h RSG组CD16表达(0.226±0.004)较Control 组(0.210±0.004)、ICH 组(0.221±0.004)和NaCl 组(0.220±0.005)的表达均显著升高(P＜0.05).脑出血造模后 24 h RSG 组 CD206 表达(0.204±0.004)较 Control 组(0.184±0.005)、ICH 组(0.195±0.005)和 NaCl 组(0.190±0.006)的表达均显著升高(P＜0.05).结论 PPARγ激动剂罗格列酮能够增强ICH后血肿周围M1、M2型小胶质细胞的表达,特别是促进小胶质细胞向M2型极化.
Abstract
Objective A model of intracerebral hemorrhage(ICH)was established to study the effect of PPARγ agonist(rosiglitazone)on the polarization of microglia(M1 and M2)around the hematoma after intracerebral hemorrhage in male SD rats.Methods A total of 48 male SD rats were divided into four groups according to the random number table method,including the normal group(Control),the intracerebral hemorrhage model group(ICH),the sodium chloride intervention group(NaCl),and the rosiglitazone intervention group(RSG),with 12 rats in each group.The ICH model of male rats was established by autologous blood.The neurological function of all groups of male rats was scored at the time points of 6 h,24 h,48 h,and 72 h.The expressions of CD16 and CD206 in the peripheral tissues of hematoma were detected by immunohistochemistry.Results The Longa scores of the ICH group were higher than those of the NaCl group at all time points(P＜0.05).The Longa scores of the RSG group were lower than those of the ICH group at all time points(P＜0.05).At 6 hours after intracerebral hemorrhage modeling,the CD16 level in the RSG group(0.226±0.004)was higher than that in the control group(0.210±0.004),the ICH group(0.221±0.004),and the NaCl group(0.220±0.005)expression was significantly increased,and the differences were statistically significant(P＜0.05).The CD206 in the RSG group(0.204±0.004)was significantly higher than that in the control group(0.184±0.005),the ICH group(0.195±0.005),and the NaCl group(0.190±0.006)at 24 hours after intracerebral hemorrhage modeling(P＜0.05).Conclusion PPARγ agonist rosiglitazone can enhance the expression of M1 and M2 microglia around he-matoma after ICH,especially promoting the transformation of microglia into M2.
维普
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