Objective This study aims to investigate the changes of TMEM88 expression in mice with acute liver injury induced by lipopolysaccharide(LPS)combined with D-galactosamine(D-GaIN),and investigate the effect of high and low TMEM88 expression on the secretion of inflammatory factors in AML-12 cells induced by LPS.Methods In vivo,20 mice were randomly divided into normal and model groups with 10 mice in each group according to random number ta-ble method.The model group was induced by LPS/D-GaIN combination,and liver tissues and blood of mice were collect-ed.In vitro,AML-12 cells were stimulated by LPS and transfected with TMEM88 to access the changes in the expression of TMEM88 and inflammatory factors.Results The model group exhibited significant liver tissue,with a successful ALI modelling rate of 80％.In addition,the hepatic TMEM88 expression level was significantly increased in the model group(P＜0.05).In AML-12 cells,the optimal condition for LPS stimulation was determined to be 100 ng/mL for 24 h.pEG-FP-C1-TMEM88 and TMEM88-siRNA were verified to be successfully transfected into LPS-stimulated AML-12 cells(P＜0.05).Furthermore,overexpression of TMEM88 resulted in an upregulation of inflammatory factors IL-1β,IL-6,and TNF-α(P＜0.01),while knockdown of TMEM88 reduced the secretion of inflammatory factors(P＜0.05).These exper-imental results suggest that the expression level of TMEM88 may influence the inflammatory process.Conclusion The expression level of TMEM88 plays an important role in the LPS/D-GaIN-induced acute liver injury model.
维普
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