Objective To establish a mouse model of exercise-induced myocardial injury caused by exhaustive exercise and to investigate the intervention effect and possible mechanism of Alpiniae oxyphylla fructus pretreatment on myocardial injury after exhaustive exercise by observing the changes of myocardial injury indexes after acute exhaustive exercise in mice,thus providing a theoretical basis and reference for further related studies.Methods Mice were randomly assigned to five different experimental groups by simple random grouping:Control group(Con,n=6),Yizhiren group(Yz,n=6),Exhaustion group(EE,n=6),Exhaustion exercise model+Yizhiren low dose group(EE+EYz,n=6),and Ex-haustion exercise model+Yizhiren high dose group(EE+HYz,n=6),all mice received specific exercise intervention and drug feeding for six weeks.The mice were sacrificed at the end of the experiment,and data on biomarkers associated with myocardial injury were collected.Results In terms of exhaustion frequency,EE+HYz group and EE+EYz group showed statistically significant difference compared with EE group(P＜0.05).Statistically significant differences were observed between EE+HYz group,Con and Yz group,EE+HYz and EE group(P＜0.05).In the analysis of biochemical parameters,the level of tumor necrosis factor α(TNF-α)in the EE+HYz group was significantly decreased compared with that in the EE group(P＜0.05).There was significant difference in erythroprotein(MYO)among the 5 groups(P＜0.05).Myocardial creatine kinase(CK-MB)was significantly different between the other 4 groups and EE group(P＜0.001).Conclusions Alpiniae oxyphylla fructus has significant anti-fatigue,anti-inflammation and protective effects on myocardial injury.By inhibiting the increase of myocardial CK-MB and MYO activity induced by exhaustive exercise,Al-piniae oxyphylla fructus reduced the degree of myocardial injury.These findings reveal the potential antioxidant effects of Alpiniae oxyphylla fructus in heart protection and may be an important strategy for the prevention and treatment of exer-cise-related myocardial injury.In addition,these results provided new research ideas for the development of intervention and treatment drugs for myocardial injury.
Alpiniae oxyphylla fructusExhaustive exerciseOxidative stressMyocardial injuryEffect and mechanism
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