Objective To explore the expression and clinical significance of Fibulin-1(FBLN1)in tongue cancer tissue and tongue cancer cells.Methods The expression of FBLN1 in head and neck cancer tissues was analyzed based on the HPA database.The association between FBLN1 and MKI67 expression in head and neck cancer was analyzed using the GEPIA database.The relationship between FBLN1 expression levels and the survival time of head and neck cancer pa-tients was examined using the Kaplan-Meier Plotter database.Gene enrichment pathway prediction was performed to in-vestigate the potential biological functions of FBLN1 in head and neck cancer.Immunohistochemistry(IHC)and Western blotting were used to validate the expression of FBLN1 in tongue cancer tissues.Stable overexpression of FBLN1 in tongue cancer cells was established,scratch and Transwell chamber experiments were conducted to assess its impact on cell inva-sion ability.Results Analysis from the HPA database showed that FBLN1 had lower expression levels in head and neck cancer tissues compared to adjacent non-cancerous tissues.Analysis from the GEPIA database revealed no significant difference(P＞0.05)in FBLN1 expression between tumor tissues and adjacent non-cancerous tissues,while MKI67 ex-hibited higher expression levels(P＜0.05)with no correlation between their expressions(P=0.280).Survival analysis from Kaplan-Meier Plotter indicated no association between FBLN1 expression and overall survival time for tongue cancer patients(log-rank P=0.190),but higher survival rates were observed when FBLN1 was highly expressed(log-rank P=0.007).Gene enrichment pathways suggested that FBLN1 may be involved in multiple pathways related to tongue cancer development.IHC staining demonstrated that both staining intensity and positive area for Fibulin-1 were significantly higher than those observed for tumor tissue samples(P＜0.001).Western blotting showed that the level of FBLN1 protein was higher in adjacent cancer tissues compared to tongue cancer tissues in 10 cases(P＜0.001).Scratch and Transwell experiments demonstrated that overexpression of FBLN1 significantly inhibits the migration and invasion ability of tongue squamous cell carcinoma cells Cal-27(P＜0.05).Conclusion FBLN1 is downregulated in tongue cancer tissues,and overexpression of FBLN1 can suppress the invasive capability of Cal-27 cells,suggesting its potential as a prognostic marker for tongue cancer patients.
Tongue cancerHead and neck cancerFibulin-1BioinformaticsCell migrationCell invasion
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