Objective Lipoyltransferase 1(LIPT1)is one of the key genes for copper death,and LIPT1 expression and its clinical significance were analysed in endometrial cancer(UCEC)using the TCGA database and clinical samples.Methods The immunohistochemical experimental results of TCGA-UCEC samples and tissue specimens of 59 UCEC pa-tients were employed to substantiate the discrepancies in LIPT1 mRNA and protein expression and their correlation with clinical characteristics,as well as prognostic analyses.Subsequently,the relationship between LIPT1 and immune cells and immune checkpoints was subjected to analysis.Results The results showed that the expression of LIPT1 gene in cancer tissues was significantly lower than that in normal tissues(P＜0.001),and it was highly expressed in the adjacent normal tissues(P＜0.001).Immunohistochemical experiments also showed that the positive expression of LIPT1 protein was lower in cancerous tissues[40.68％(24/59)]than in paracancerous tissues[76.92％(20/26),X2=9.495,P=0.002].The differences in age(P=0.025),pathological grade(P=0.004)and pathological type(P＜0.001)of pa-tients with different LIPT1 expression levels were statistically significant.The analysis of the clinical samples showed that the differences in age and pathological grading of patients with different LIPT1 expression levels were statistically signifi-cant(P＜0.05).Patients with UCEC who exhibited high LIPT1 expression exhibited poorer OS,DSS and PFI,which were identified as independent poor prognostic factors for OS.LIPT1 expression was found to be correlated with CD8+T-cells,macrophages and neutrophils(P＜0.05).The expression of LIPT1 was correlated with PD-L1(P＜0.01),but not correlated with PD-1 or CTLA-4.Conclusion LIPT1 is expressed at low levels in UCEC,but high levels are associated with poor prognosis and may be related to cancer cell immune escape.It is anticipated that this will become a new thera-peutic target for UCEC.
Uterine corpus endometrial carcinomaLipoyltransferase 1Poor prognosisTherapeutic target
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NSTL
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