Teaching practice of real-time PCR applied in the discussion group experiment
Real time quantitative PCR,as an important technology in life sciences and related fields,has become an indispensable knowledge content in biological experimental teaching in universities.Based on the basic teaching content of molecular biology experiments,the course group has conducted a comprehensive experiment targeting undergraduate students around real-time quantitative PCR tech-nology.Fully considering the students'theory and operation level and the characteristics of the under-graduate experiment teaching,the course group has optimized the experimental design and methods,meanwhile,adopted the discussion group teaching model,which has improved the teaching efficiency and teaching quality,highlighted the main position of students in the experimental teaching,and helped to cultivate students'scientific thinking,scientific literacy and comprehensive practical ability.