Comprehensive experimental design based on quorum sensing regulation of Shewanella
In this paper,a new control strategy experiment targeting the quorum sensing system of a-quatic spoilage bacteria is designed to explore the regulation of quorum sensing quenching enzyme AiiA on quorum sensing eavesdropping of Shewanella and the effects on its growth,biofilm and protease.The results indicate that the QS eavesdropping and its related metabolic activities are significantly influ-enced by the recombinant AiiA both in the co-culture model and the C6-HSL and O-C6-HSL simula-ted model.The experiment relies on the resources of scientific research,integrates frontier science and technology,and combines the professional experimental teaching of genetic engineering,enzyme engi-neering and food storage principle.It has comprehensive and innovative characteristics,and helps to cultivate students'learning interest,knowledge integration ability,exploration spirit and hands-on op-eration skills.
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