The existing method of desalting column in proteomic sample pre-treatment has many steps and is prone to loss of trace samples.Here,a proteomic pre-treatment method of enzymatic digestion assisted by ultrafiltration tube and desalting by heating is proposed.Specifically,the buffer was re-placed with ammonium bicarbonate solution by centrifugation before enzymatic digestion using ultrafil-tration tube assistance,and the ammonium bicarbonate in the peptide solution was removed by heating after enzymatic digestion to finally obtain the desalted pure peptides.This method is easy to operate without loss of sample and does not require an elution step,and can be easily interfaced directly with LC-MS for further mass spectrometry analysis.
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