To investigate the combustion process of diesel,natural gas,and hydrogen in the engine cylinder,a diesel/natural gas/hydrogen(DNH)tri-fuel mechanism containing 120 species and 612 steps of elementary reactions was constructed.The ignition delay sensitivity analysis and reaction path analysis were carried out on the DNH mechanism,the key reaction preexponential factors were adjusted,and the ignition delay time and laminar flame speed of the DNH mechanism were verified by Chemkin.Meanwhile,the three combustion modes of pure diesel,diesel/natural gas,and diesel/natural gas/hydrogen in the engine cylinder were verified by the method of computational fluid dynamics coupling DNH kinetic mechanism.The results show that the predicted results of the DNH mechanism constructed based on the high carbon number multi-component diesel model are in good agreement with the experimental data of ignition delay and laminar flame velocity.The calculation results of DNH mechanism coupled with computational fluid dynamics are consistent with the changes of pressure and heat release rate in the engine cylinder measured by experiments,and the DNH mechanism is suitable for predicting the in-cylinder combustion process of a tri-fuel engine.