Associations of the apolipoprotein C3 rs2854116,rs2854117 and rs5128 single nucleotide polymorphisms with metabolic syndrome
Objective To explore the relationships of single nucleotide polymorphisms(SNPs)at rs2854116,rs2854117 and rs5128 loci of the apolipoprotein C3(APOC3)gene with metabolic syndrome(MS)in Chinese Han population.Methods Based on a representative survey on the status of nutrition and health among urban and rural residents in Nanjing City,a case-control study matched by gender and age was conducted.Peripheral blood leukocyte DNA was extracted to determine SNPs at each locus.After adjusting the demographic characteristics and lifestyle factors,multivariate unconditional logistic regression was used to analyze the relationships of SNPs at rs28541 16,rs28541 17 and rs5128 loci of the APOC3 gene with MS.Results The cases enrolled were divided into the MS group and the control group(each n=253).No statistically significant differences were found in the composition of demographic characteristics between the two groups(P＞0.05)except for education level and family history of hypertension.The SNPs at rs2854116,rs2854117 and rs5128 loci were correlated with MS in both codominant(OR=2.219,95％CI:1.163-4.234;OR=2.619,95％CI:1.336-5.136;OR=4.152,95％CI:1.651-10.442)and dominant models(OR=1.782,95％CI:1.083-2.934;OR=1.721,95％CI:1.064-2.783;OR=1.702,95％CI:1.048-2.763).The SNPs at rs2854117 and rs5128 loci were correlated with MS in recessive model(OR=2.054,95％CI:1.131-3.731;OR=4.925,95％CI:1.860-13.043).There was a linkage imbalance among the loci.The frequencies of C-T(x2=7.061,P=0.008),T-C(x2=17.365,P＜0.00l)and T-T(x2=4.208,P=0.040)haplotypes at rs2854117-rs2854116,C-G(x2=10.720,P=0.001),T-C(x2=14.911,P＜0.001)haplotypes at rs2854116-rs5128,and C-T-C(x2=13.271,P＜0.001)and T-C-G(x2=14.896,P＜0.001)haplotypes at rs2854117-rs2854116-rs5128 showed statistically significant differences between the MS group and the control group.The three-order interaction model of rs2854116,rs2854117 and rs5128 was the best,and the cross-validation agreement was 10/10,with statistically significant differences(P=0.011).Conclusion The SNPs at rs2854116,rs2854117 and rs5128 loci of the APOC3 gene are associated with MS incidence in Chinese Han population,and there may be interaction among these loci.
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