Status of Clostridium perfringens infection in patients with foodborne diarrhea in Yinzhou District,Ningbo City
Objective To understand the infection status of Clostridium perfringens(C.perfringens)in patients with foodborne diarrhea in Yinzhou District of Ningbo City so as to provide a basis for its prevention and control.Methods The clinical fecal samples of patients with foodborne diarrhea in Yinzhou District of Ningbo City were collected,and the genomic DNA was extracted directly.Fluorescence quantitative PCR was used to detect the cpa-gene of C.perfringens,and then we detected the cpe and cpb2 genes in positive samples.The cpa-positive was used as the indicator of C.perfringens infection.We analyzed the status of C.perfringens detection and its co-infection with other foodborne diarrhea pathogens in patients with different characteristics of foodborne diarrhea.Results A total of 99 samples of C.perfringens cpa gene were detected in 534 samples(18.54％,99/534),and the detection rates of cpe and cpb2 in cpa-positive samples were 15.15％and 78.79％respectively.No statistically significant difference was found in the detection rate of C.perfringens between male and female patients with foodborne diarrhea(X2=2.765,P＞0.05),but the detection rate showed a downward trend with the increasing age(x2trend2=12.277,P＜0.001).Foodborne diarrhea caused by C.perfringens was common throughout the year,but there was no statistically significant difference in the detection rate among months(x2=6.926,P＞0.05).No statistically significant difference was observed in the detection rate of C.perfringens among patients with foodborne diarrhea caused by different suspected exposure foods(x2=6.856,P＞0.05)and food processing methods(x2=12.329,P＞0.05).Among the C.perfringens positive samples detected,24 samples were co-infected with norovirus,rotavirus,Salmonella,Vibrio parahaemolyticus and other foodborne pathogens.Conclusion C.perfringens is one of the main pathogens of foodborne diarrhea in Yinzhou District of Ningbo City,and there is mixed infection.Special attention should be paid to the etiological surveillance and prevention of C.perfringens.
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