Pathogenic spectrums of 596 patients with acute respiratory tract infections in Wuhan City,2020-2021
Objective One year after the outbreak of the SARS-CoV-2 infection,the composition of pathogenic spectrums of patients with respiratory tract infections in Wuhan in winter was clarified under non-drug intervention measures,and the etiological basis for prevention and treatment of respiratory tract infectious diseases was provided.Methods We collected throat swab samples from acute respiratory tract infection(ARTI)cases in fever clinics of eight large hospitals in Wuhan from December 28,2020 to February 14,2021.Nucleic acid extraction was performed using an automatic nucleic acid extractor,and real-time fluorescence quantitative PCR technology was used to detect 23 respiratory pathogens.Microsoft Office Excel 2016 was used to input and organize the data,and then a database was established.Statistical analysis was performed with SPSS 20.0 software.Results A total of 596 ARTI cases were collected,including 335 males and 261 females,with a male-to-female ratio of 1.28:1.The youngest was 5 months old and the oldest 91 years old.The total positive rate of 23 respiratory pathogens was 86.24％(514/596),and a total of 13 pathogens of 23 respiratory pathogens were positive.The top three pathogens were Streptococcus pneumoniae(SPN)(30.47％,323/1,060),Klebsiella pneumoniae(KPN)(24.25％,257/1,060)and Haemophilus influenzae(HIN)(19.43％,206/1,060).Among the 514 positive cases,331 were mixed infections,accounting for 64.40％(331/514).The proportion of mixed infection between bacteria(54.68％,181/331)was higher than that of bacteria and virus(45.01％,149/331).Among the 331 cases of mixed infections,only one case of mixed infection between human metapneumovirus(HMPV)and respiratory syncytial virus(RSV)was detected,accounting for 0.30％(1/331).There were statistically significant differences in the detection rates of HIN,KPN,SPN,HMPV,RSV,respiratory adenovirus(RA),human bocavirus(HBoV)and human enterovirus(HEV)in patients of different ages as well as in the detection rates of HIN,KPN,SPN,HMPV and RSV in patients of different body temperatures.No statistically significant differences were found in the detection rates of all pathogens between male and female patients.The detection rates of group A streptococcus(GAS)and RSV between national and non-national influenza sentinel hospitals showed statistically significant differences.Conclusion SPN,KPN,HIN,HMPV and Staphylococcus aureus(SA)were the main respiratory pathogens in Wuhan in winter under non-drug intervention measures.Strengthening monitoring and prevention of these pathogens is the focus of prevention and control of respiratory tract infections in Wuhan in winter.
acute respiratory tract infectionnon-drug intervention measurerespiratory pathogenpathogen spectrummonitoring and prevention
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