Objective To explore the effect of different repair methods on improving the nuclear gray staining caused by poor fixation of HE staining and improve the quality of HE staining.Methods Seven cases of gastrointestinal endoscopic biopsy tissues with nuclear gray staining caused by poor fixation were collected,all of them were re-sliced and repaired with six different Processing methods.Results The six methods all could improve the problem of gray nuclei and make Helicobacter pylori in tissues easier to be observed,but the high temperature and high pressure repair effect of PBS buffer and citric retrieval solution were better,EDTA made nuclear extrusion and deformation,cytoplasm destroyed.Conclusion High temperature and high pressure repair effect of PBS buffer and citric retrieval solution were better to improve the problem of gray nuclei and make Helicobacter pylori in tissues easier to observe.
HE stainingGray nucleiRepair methodsHelicobacter pylori
维普
万方数据
