首页|化学发光法与甲苯胺红不加热血清试验在梅毒检测中的应用价值

化学发光法与甲苯胺红不加热血清试验在梅毒检测中的应用价值

Application value of chemiluminescence and toluidine red unheated serum test in syphilis detection

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目的 分析在梅毒检测中化学发光法与甲苯胺红不加热血清试验2种方式的实际应用价值.方法 采用回顾性研究方式,对逆向梅毒筛查流程实验室数据进行分析,对2022年1月至2023年6月入院的867例患者进行化学发光法进行抗体筛查试验,检测显示阳性样本实施非梅毒螺旋体抗原血清甲苯胺红不加热试验检测进行复测,分析2种检测方法对梅毒检出结果.结果 在867例样本中,采用化学发光法检测显阳性样本6例,对6例阳性样本采用甲苯胺红不加热试验进行复测,结果呈阳性4例,阴性2例.化学发光法标本的吸光度与临界值的比值(S/CO)>10时,采用甲苯胺红不加热血清试验具有较高的复查阳性率.结论 梅毒检测中化学发光法的检出率较高,已经确诊的梅毒患者需要甲苯胺红不加热血清试验进行滴度测定,来观察疗效、随访及监测有无复发等.综合使用多种检测方法,可以提高梅毒的检测的准确性,更好地指导患者的治疗和管理.
Objective To analyze the practical application value of chemiluminescence method and tolui-dine red unheated serum test in syphilis detection.Methods A retrospective study was conducted to analyze the laboratory data of the reverse syphilis screening process.867 patients admitted from January 2022 to June 2023 were tested for antibody screening by chemiluminescence method.The positive samples were retested by non-Tre-ponema pallidum antigen serum Toluidine Red unheated test.The detection results of syphilis by the two methods were analyzed.Results Among the 867 samples,6 cases were positive by chemiluminescence detection,and 6 cases were retested by toluidine red unheated test.The results were positive in 4 cases and negative in 2 cases.When the S/CO value of the chemiluminescence method was>10.the toluidine red unheated serum test had a high positive rate of re-examination.Conclusion The patients underwent antibody screening test by chemilumines-cence method,and the positive samples were detected by non-Treponema pallidum antigen serologic test.The de-tection rate of chemiluminescence method is high.The confirmed syphilis patients still need toluidine red unheat-ed serum test for titer determination to observe the curative effect,follow-up and monitor whether there is recur-rence.A variety of detection methods are used comprehensively,it can improve the accuracy of syphilis detection and better guide the treatment and management of patients.

SyphilisLuminescent measurementsToluidine red unheated serum testPositive rate

谢乔芬、赖谋忠、杨未琴

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井冈山市第二人民医院检验科,井冈山 343609

梅毒 发光测定法 甲苯胺红不加热血清试验 阳性率

2024

实用医技杂志
山西医药卫生传媒集团有限责任公司

实用医技杂志

影响因子:0.534
ISSN:1671-5098
年,卷(期):2024.31(4)