Objective To study a new method of tissue preservation and preparation in immunohisto-chemical positive control and make the method of preparation of immunohistochemical positive control easier and more efficient.Methods Take pleuroperitoneal fluid as a specimen,centrifuge pleuroperitoneal fluid and then to break red blood cells,collect sedimentary cells,then dehydrate the cells,mix the cells with paraffin wax and liq-uid paraffin and then pour them into lip balm tube,smear it on adhesive glass slide,and then the cells in the cream can be adhered to the glass slide and use immunohistochemical stains finally.Results Using microscopes to observe cells,the cells are evenly distributed,and the shape of cells are integral.In HE staining,the nucleus and cytoplasm staining contrast are clear,the nucleus were purple with hematoxylin,the cytoplasm was pale pink with eosin,and the histiocytes,lymphocytes and mesothelial cells are clearly identifiable.In immunohistochemical CD68 staining,the nucleus of histiocytes were stained brown.In immunohistochemical Ki-67 staining,the nu-clear of cells in proliferation phase were stained brown.Immunohistochemical staining results showed clear ex-pression and accurate localization.Conclusion The tube of immunohistochemical positive control tissue can re-place traditional preparation of positive control,the tube of immunohistochemical positive control tissue can be used in the process of positive control preparation
万方数据
维普
