Liquid-based thin-layer cytology combined with second-generation hybridization capture assay for detection of high-risk human papillomavirus testing in cervical cancer screening
Liquid-based thin-layer cytology combined with second-generation hybridization capture assay for detection of high-risk human papillomavirus testing in cervical cancer screening
Objective To explore the efficacy of liquid-based thin-layer cytology(TCT)combined with second-generation hybridization capture assay for the detection of high-risk human papillomavirus(HPV-DNA)test for the diagnosis of cervical cancer.Methods From January 2020 to December 2022 one hundred and sixteen suspected cervical cancer patients admitted to our hospital were selected for the study.All the patients were subjected to TCT,second-generation hybridization capture method for detecting high-risk HPV-DNA detection as well as pathological biopsy,and the efficacy of the two diagnostic methods of TCT and second-generation hybridization capture method for detecting high-risk HPV-DNA detection,both singly and in combination,was analyzed with the diagnostic results of the pathological biopsy as the gold standard.Results The detection efficacy of high-risk HPV-DNA by TCT and second-generation hybridization capture method was similar(P＞0.05);compared with the single diagnosis of the two diagnostic methods,the sensitivity,negative predictive value and accuracy of the combined diagnosis were higher(P＜0.05),and the rate of missed diagnosis and misdiagnosis were lower(P＜0.05).The specificity and positive predictive value of the single diagnosis and combined diagnosis of high-risk HPV-DNA by TCT and second-generation hybridization capture method were similar(P＞0.05).Conclusion TCT and second-generation hybridization capture assay for high-risk HPV-DNA detection have good diagnostic efficacy in cervical cancer screening,and their combined diagnosis can further improve diagnostic precision and sensitivity.
关键词
人乳头瘤病毒/宫颈肿瘤/液基细胞学
Key words
Human papillomavirus/Uterine cervical neoplasms/Liquid-based cytology
维普
万方数据