Objective To evaluate the value of matrix assisted laser desorption ionization time of flight mass spectrometry(MALDI-TOF MS)in detecting carbapenem resistant Klebsiella pneumoniae(CRKP).Methods One hundred and eight strains of Klebsiella pneumoniae isolated from in-patients in our hospital from January 2020 to January 23 were collected.CRKP and carbapenem sensitive Klebsiella pneumoniae(CSKP)were screened out with imipenem or meropenem cards.The drug sensitivity of CRKP was judged by CLSI m100-S27,and the drug resistance gene sequence of CRKP was detected with polymerase chain reaction(PCR),the MALDI-TOF MS was used to detect characteristic peaks of CRKP and carbapenem sensitive Klebsiella pneumoniae(CSKP).The characteristic peaks of CRKP and CSKP was verified by 100 strains of Klebsiella pneumoniae collected at the same time.Results Among the 108 strains of Klebsiella pneumoniae,25 strains were CRKP and 83 strains were CSKP.All 25 strains of CRKP were resistant to carbapenems,cephalosporins,aztreonam,quinolones and aminoglycosides to varying degrees.Eighty-three strains of CSKP were sensitive to almost all types of antibiotics.Twenty-five CRKP strains were detected to carry carbapenemase genes.The sequencing results showed that 20 strains(80.0％)carried the blaKPC gene,3 strains(12.0％)carried the blaNDM gene and 2 strains(8.0％)carried the blaOXA-48 gene.No blaGES,blaIMP,blaNDM or blaVIM were detected.The characteristic peaks of CRKP were 4 159.5 m/z,8 369.7 m/z and 10 808.0 m/z,respectively,while the characteristic peaks of CSKP were 3599.6 m/z and 10 087.5 m/z,respectively.One hundred Klebsiella pneumoniae strains were collected and detected by characteristic peaks of CRKP and CSKP from MALDI-TOF MS.Among 100 Klebsiella pneumoniae strains,30 CRKPs were detected 28 CRKPs and 70 CSKPs were detected 68 CSKPs by MALDI-TOF MS,with an accuracy of 96.0％(96/100).Conclusion The resistance genes of CRKP in this region are mostly blaKPC,and MALDI-TOF MS can quickly detect CRKP.The characteristic peaks of CRKP are 4 159.5 m/z,8 369.7 m/z and 10 808.0 m/z,respectively.
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