一种新型宫颈液基细胞标本制作细胞蜡块的方法
A new method for preparing cell paraffin blocks from a cervical liquid-based cytology specimen
丁诗情1
作者信息
- 1. 浙江大学医学院附属第四医院病理科,义乌 322000
- 折叠
摘要
目的 寻找一种减少细胞损失,提高诊断准确率的宫颈液基标本细胞蜡块制作方法.方法 收集液基薄层细胞学检查(TCT)诊断为高级别鳞状上皮内瘤变(HSIL)、低级别鳞状上皮内瘤变(LSIL)的妇科宫颈液基细胞标本各30例,用新型的方法制作细胞蜡块,行苏木精-伊红(HE)染色和P16、Ki-67免疫组织化学染色,对比与TCT的符合率,再将此方法运用于非典型鳞状细胞-不能除外高级别鳞状上皮内瘤变(ASC-H).结果 高级别鳞状上皮内瘤变细胞蜡块与TCT诊断符合率为100％(30/30),低级别鳞状上皮内病变细胞蜡块与TCT诊断符合率为93.3％(28/30),差异无统计学意义(P＞0.05).结论 此种细胞蜡块制作方法制作的细胞蜡块与TCT诊断结果符合率高,可用于ASC-H的进一步诊断.
Abstract
Objective To explore a method for making cell paraffin blocks from liquid-based cervical cytology specimens in order to reduce cell loss and improve diagnostic accuracy.Methods Thirty cases each of gynecological cervical liquid-based cytology specimens diagnosed by Thin-layer cervical cytology test(TCT)as high-grade squamous intraepithelial lesion(HSIL)and low-grade squamous intraepithelial lesion(LSIL)were collected.A novel method was used to prepare cell paraffin blocks,and HE staining along with P16 and Ki-67 immunohistochemical staining were performed.The consistency rate with TCT was compared,and this method was further applied to atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesions(ASC-H).Results The consistency rate between the high-grade squamous intraepithelial lesion cell paraffin blocks and TCT diagnosis was 100％,and the consistency rate between the low-grade squamous intraepithelial lesion cell paraffin blocks and TCT diagnosis was 93.3％,with no statistical significance(P＞0.05).Conclusion The cell paraffin block preparation method proposed here has a high consistency rate with TCT diagnostic results and can be used for further diagnosis of atypical squamous cells-cannot exclude high-grade squamous intraepithelial lesions.
关键词
子宫颈,蜡块/液基薄层细胞学检查Key words
Cervix ulteri/Paraffin block/Thin-prep cytology test引用本文复制引用
出版年
2024
NETL
NSTL
万方数据