Objective To assess the impact of storage duration and temperature on the detection intensity of PD-L1(22C3).Methods Tissue microarrays were constructed using well-preserved samples of normal tonsil,placental tissue,and oral squamous cell carcinoma tissue,all known to exhibit PD-L1 positivity.A total of 36 gelatinized white tissue chips were excised and subsequently divided into four groups,with an average of nine white tissue samples per group.Each group contained a mix of the three tissue types.The first and second groups did not bake their slices,where as the third and fourth groups baked theirs in an oven at(58±2)℃ for 30 minutes.Subsequently,the first and third groups stored their slices in a refrigerator set at room temperature(25 ℃),while the second and fourth groups placed theirs in a refrigerator at-20℃.Results Among the tonsil crypt epithelial cells,placental tissue,and tumor cells of oral squamous cell carcinoma in the four groups of chips,the positive intensity of the first and third groups of chips decreased significantly,while the positive intensity of the second and fourth groups of chips did not decrease significantly.Comparing the four groups of chips pairwise,it can be concluded that the positive intensity of the four groups of chips under the same temperature conditions remains unaffected regardless of whether they are baked or not.The positive intensity of chips stored at-20 ℃ is not significantly reduced compared to those stored at room temperature(25 ℃),indicating better preservation of antigens.Conclusion The detection of PD-L1 is influenced by storage duration and temperature.
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