Objective To characterize the bacterial profile presenting in peripheral blood of patients with severe acute pancreatitis(SAP)and the correlation between bacterial translocation and pancreatic infection.Methods The peripheral blood samples were collected from 45 patients whose pancreatic fluid samples were collected for routine microbiological test from 2020 to 2021 in General Hospital of Eastern Theater Command.Genomic DNA was extracted from peripheral blood sample,and detected using the 16S rRNA gene next-generation sequencing(NGS).Results Seventeen peripheral blood samples collected from 15 severe pancreatitis patients were successfully determined using 16S rRNA gene NGS.Fifty-seven bacterial strains were detected which belongs to 27 species in 25 genera.The detection rates of Mycobacterium aubagnense and Acinetobacter johnsonii were 52.9％(9/17)and 35.3％(6/17),respectively.Compared with the results of corresponding pancreatic fluid samples,the bacterial profile of peripheral blood was significant different.Only two bacterial strains were detected in both peripheral blood and pancreatic fluid.Conclusion There is significant difference on bacterial profile between in the peripheral blood and in the pancreatic fluid samples for SAP patients.Hematogenous dissemination may not be the main way for bacterial translocation.
维普
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