摘要
目的 研究重症胰腺炎(SAP)患者外周血细菌谱,探讨细菌易位与胰腺感染之间的相关性.方法 收集东部战区总医院2020-2021年45例SAP患者外周血标本,外周血标本和胰液常规检测标本采集时间为同一天.提取标本DNA,采用聚合酶链反应(PCR)扩增,进行16S rRNA高通量测序(NGS).结果 来自15例SAP患者的17例次外周血标本成功进行了 16S rRNA NGS检测,并检测到了 57株细菌,分属于25个属27个种.奥巴涅分枝杆菌和约氏不动杆菌检出率分别为52.9％(9/17)和35.3％(6/17).与相应胰液标本的检测结果相比,两者具有极大的差异性,仅在2对标本(胰液和外周血)中检测2个相同的细菌.结论 SAP患者外周血与胰腺感染的细菌种类可能存在极大的差异,血液循环可能不是细菌易位的主要途径.
Abstract
Objective To characterize the bacterial profile presenting in peripheral blood of patients with severe acute pancreatitis(SAP)and the correlation between bacterial translocation and pancreatic infection.Methods The peripheral blood samples were collected from 45 patients whose pancreatic fluid samples were collected for routine microbiological test from 2020 to 2021 in General Hospital of Eastern Theater Command.Genomic DNA was extracted from peripheral blood sample,and detected using the 16S rRNA gene next-generation sequencing(NGS).Results Seventeen peripheral blood samples collected from 15 severe pancreatitis patients were successfully determined using 16S rRNA gene NGS.Fifty-seven bacterial strains were detected which belongs to 27 species in 25 genera.The detection rates of Mycobacterium aubagnense and Acinetobacter johnsonii were 52.9％(9/17)and 35.3％(6/17),respectively.Compared with the results of corresponding pancreatic fluid samples,the bacterial profile of peripheral blood was significant different.Only two bacterial strains were detected in both peripheral blood and pancreatic fluid.Conclusion There is significant difference on bacterial profile between in the peripheral blood and in the pancreatic fluid samples for SAP patients.Hematogenous dissemination may not be the main way for bacterial translocation.
维普
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