Objective To explore the application of a conical-bottomed specimen bottle containing eosin fixative in improving endocervical curettage(ECC)preparation.Methods We used a conical-bottomed specimen bottle containing eosin fixative to collect ECC specimens.Before sampling,the specimen bottle was directly centrifuged at 4 000 r/min for 10 min.The supernatant was discarded,and all tissues were scraped with tweezers or long-handled ear spoons.ECC specimens were prepared according to the standard protocol.Results After high-speed centrifugation,small red clots could be seen at the bottom of the conical-bottomed specimen bottle or the wall,which made it easy to get the specimen.After routine preparation,the tissue or cells were concentrated under the microscope,without degeneration or shrinkage.The staining was clear with good contrast.Cervical mucous gland epithelial cells,inflammatory cells,and intact cervical canal mucosa could be observed under the microscope.It had no effect on the results of immunohistochemistry.It was easy to determine the morphology of cervical epithelial cells and lesions,which met pathological diagnosis.Conclusion The use of conical-bottom specimen bottles containing eosin fixative to prepare ECC specimens can maximize the retention of tissues and cells in the specimens.This method is convenient and practical,and the effect is good.
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