Objective To explore a simple and efficient method for producing cell wax blocks through experiments.Methods Divide the sample into two groups:the first group takes the sample and places it in a centrifuge tube,centrifuge and discard the supernatant.Wrap the cell sediment with tweezers in a lens cleaning paper and place it in an embedding box to make a cell wax block.The second group took samples and centrifuged to discard the supernatant.BD Cytorich Red Preservative was added to the centrifuge tube,and the cell sediment was blown and mixed before centrifuging to discard the supernatant.The centrifuge tube was placed in a-20℃ freezer for 20 minutes,and the cell sediment was wrapped in lens cleaning paper with tweezers and placed in an embedding box to make cell wax blocks.Results Group 1:The blood clot in the sample was not properly processed,and the cell sediment adhered to the centrifuge tube wall,forceps,etc.,resulting in loss of cellular components.The sample needs to be resubmitted for testing.During routine slicing,the slices are incomplete and show knife marks.During HE staining,the structure is incomplete,and during immunohistochemical staining,background staining occurs,which affects the interpretation of the results.Group 2:The blood clots in the sample were cleaned up,and the cell sediment did not adhere to the centrifuge tube wall.The cell components could be completely collected,and the slices were smooth without knife marks during routine sectioning.The structure was intact during HE staining,and the red blue contrast was obvious.Immunohistochemical staining showed accurate localization of positive cells without background staining.Conclusion This method of making cell wax blocks significantly improves the quality of cell wax block production and the detection rate of tumor cells.The entire operation process is simple,efficient,and cost-effective,which is worth recommending.
维普
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