Levels and significances of T lymphocyte subsets in the peripheral blood and cancer tissues of glioma patients
Objective To observe the changes of T lymphocyte subsets in the peripheral blood and cancer tissues of glioma patients,and to explore its role in glioma.Methods Twelve patients with glioma(glioma group)were diagnosed and treated in the Second Affiliated Hospital of Xinjiang Medical University from December,2022 to August,2023,and were divided into low-grade group(n=2)and high-grade group(n=10)according to the WHO classification.All patients received surgery,the peripheral blood samples were collected before surgery,and the cancer tissues and adjacent normal tissues were harvested during surgery.Another healthy volunteers were collected the peripheral blood samples on the day of physical examination.The flow cytometry was used to detect the percentages of CD3+,CD4+and CD8+T lymphocytes in the peripheral blood,cancer tissues and adjacent normal tissues,and CD4+/CD8+was calculated.Immunohistochemistry was used to detect the expression of Ki-67 in the cancer tissues.The levels of T lymphocyte subsets were compared in the peripheral blood between glioma group and control group,between cancer tissues and adjacent normal tissues and between peripheral blood and cancer tissues in glioma group,and in the peripheral blood and cancer tissues between low-grade group and high-grade group.Pearson correlation method was used to analyze the correlation between T lymphocyte subsets and Ki-67 expression in glioma patients.Results The percentage of CD4+T lymphocytes in the peripheral blood was lower in glioma group[(41.41±9.16)％]than that in control group[(50.74±0.69)％](t=3.407,P=0.007),and there were no significant differences in the percentages of CD3+and CD8+T lymphocytes and the CD4+/CD8+between two groups(P＞0.05).The percentages of CD4+and CD8+T lymphocytes were lower in the cancer tissues[(58.04±13.76)％,(16.47±9.56)％]than those in the adjacent normal tissues[(77.43±24.82)％,(25.48±9.01)％]in glioma group(t=5.516,P=0.004;t=2.157,P=0.007),and there were no significant differences in the percentage of CD3+T lymphocytes and CD4+/CD8+(P＞0.05).The percentage of CD4+T lymphocytes in the peripheral blood was lower in high-grade group[(43.95±11.53)％]than that in low-grade group[(49.60±43.95)％](t=2.422,P=0.009),the percentage of CD8+T lymphocytes was higher in high-grade group[(29.63±12.48)％]than that in low-grade group[(22.30±3.59)％](t=2.645,P=0.016),and the percentage of CD3+T lymphocytes and CD4+/CD8+showed no significant differences(P＞0.05).The percentages of CD4+and CD8+T lymphocytes were lower in high-grade group[(53.80±11.45)％,(13.90±2.81)％]than those in low-grade group[(72.90±3.54)％,(28.30±5.52)％](t=2.576,P=0.003;t=3.579,P=0.008),CD4+/CD8+was higher in high-grade group(3.87±0.04)than that in low-grade group(2.64±0.64)(t=0.645,P=0.030),and there was no significant difference in the percentage of CD3+T lymphocytes between two groups(P＞0.05).The percentages of CD3+and CD8+T lymphocytes were lower in the cancer tissues than those in the peripheral blood in glioma group(P＜0.05),and the percentage of CD4+T lymphocytes and CD4+/CD8+were higher in the cancer tissues than those in the peripheral blood(P＜0.05).The percentage of CD3+T lymphocytes in glioma group was negatively correlated with the expression of Ki-67(r=-0.669,P=0.024),the percentage of CD4+T lymphocytes was positively correlated with the expression of Ki-67(r=0.681,P=0.009),and the percentage of CD8+T lymphocytes and CD4+/CD8+were not correlated with Ki-67(r=-0.497,P=0.120;r=0.162,P=0.634).Conclusions The levels of T lymphocyte subsets in the peripheral blood and cancer tissues of glioma patients are out of balance,which is mainly demonstrated by the decease of percentage of CD4+T lymphocytes.CD4+T lymphocytes are mostly distributed in the cancer tissues,and CD8+T lymphocytes are mostly distributed in the peripheral blood.The cancer cells proliferate more rapidly in glioma patients with a low percentage of CD4+T lymphocytes.
NETL
NSTL
万方数据
