Correlation between serum miR-363-3p expression and clinical pregnancy after ovulation induction therapy in infertile patients with polycystic ovary syndrome
Objective To observe the changes of serum miR-363-3p expression in infertile patients with polycystic ovary syndrome(PCOS),and to explore its relationship with clinical pregnancy after ovulation induction therapy.Methods Totally 115 infertile PCOS patients(PCOS group)were diagnosed and treated in the First People's Hospital of Nanyang from January,2019 to December,2021,and another 60 patients without PCOS were diagnosed male infertile(control group).Real-time fluorescence quantitative PCR was used to detect the relative expression of serum miR-363-3p on the next day after admission.The age,body mass index,blood pressure,fasting plasma glucose,fasting insulin,insulin resistance index,sex hormone,anti-Müller's hormone,and blood lipid were compared between two groups.Pearson correlation method was used to analyze the correlations of the relative expression of miR-363-3p with fasting insulin,insulin resistance index,sex hormone,anti-Müller's hormone and blood lipid in PCOS patients.All patients in PCOS group were given standardized ovulation induction therapy for 3 courses,and were divided into clinical pregnancy group(n=67)and non-pregnancy group(n=48).The age,body mass index,blood pressure,fasting plasma glucose,fasting insulin,insulin resistance index,sex hormone,anti-Müller's hormone,blood lipid and relative expression of serum miR-363-3p were compared between clinical pregnancy group and non-pregnancy group.Multivariate logistic regression analysis was done to evaluate the influencing factors of clinical pregnancy failure in PCOS patients after ovulation induction therapy.Results(1)The insulin resistance index,fasting insulin,luteinizing hormone,follicle stimulating hormone,testosterone,prolactin,progesterone and anti-Müller's hormone were higher in PCOS group[2.86±0.58,(25.63±2.51)mu/L,(14.20±3.49)u/L,(8.32±1.47)u/L,(0.75±0.21)μg/L,(14.85±2.69)μg/L,(1.21±0.41)nmol/L,(9.54±2.46)μg/L]than those in control group[1.92±0.41,(12.40±1.24)mu/L,(5.63±0.74)u/L,(5.74±1.05)u/L,(0.42±0.14)μg/L,(10.32±3.51)μg/L,(0.75±0.36)nmol/L,(3.68±1.58)μg/L](P＜0.05).The level of estradiol and the relative expression of miR-363-3p were lower in PCOS group[(106.41±26.47)pmol/L,0.46±0.09]than those in control group[(163.58±32.51)pmol/L,1.00±0.18](P＜0.05).There were no significant differences in the age,body mass index,blood pressure,fasting plasma glucose,total cholesterol,triacylglycerol,high-density lipoprotein cholesterol,and low-density lipoprotein cholesterol between PCOS group and control group(P＞0.05).The relative expression of miR-363-3p in PCOS patients was negatively correlated with the fasting insulin(r=-0.753,P＜0.001),insulin resistance index(r=-0.742,P＜0.001),luteinizing hormone(r=-0.635,P＜0.001),follicle stimulating hormone(r=-0.596,P＜0.001),testosterone(r=-0.658,P＜0.001),prolactin(r=-0.584,P＜0.001),progesterone(r=-0.695,P＜0.001)and anti-Müller's hormone level(r=-0.684,P＜0.001),was positively correlated with the estradiol level(r=0.748,P＜0.001),and was not correlated with the age,body mass index,blood pressure,fasting plasma glucose,total cholesterol,triacylglycerol,high-density lipoprotein cholesterol and low-density lipoprotein cholesterol.(2)The body mass index,fasting insulin,insulin resistance index,luteinizing hormone,follicle stimulating hormone,testosterone and anti-Müller's hormone were lower in clinical pregnancy group[(22.01±1.57)kg/m2,(24.01±1.87)mu/L,2.30±0.36,(12.04±3.41)u/L,(7.41±1.24)u/L,(0.59±0.15)μg/L,(12.15±1.65)μg/L]than those in non-pregnancy group[(26.30±2.01)kg/m2,(27.32±1.65)mu/L,2.94±0.45,(17.62±3.21)u/L,(9.63±1.02)u/L,(0.83±0.34)μg/L,(7.69±1.85)μg/L](P＜0.05).The level of estradiol and the relative expression of miR-363-3p were lower higher in clinical pregnancy group[(114.63±15.64)pmol/L,0.64±0.12]than those in non-pregnancy group[(101.32±18.42)pmol/L,0.25±0.05](P＜0.05).There were no significant differences in the age,blood pressure,fasting plasma glucose,prolactin,progesterone,total cholesterol,triacylglycerol,high-density lipoprotein cholesterol and low-density lipoprotein cholesterol between two groups(P＞0.05).Insulin resistance index(OR=3.823,95％CI:1.845-6.418,P＜0.001),luteinizing hormone(OR=5.778,95％CI:1.458-9.326,P＜0.001),testosterone(OR=2.617,95％CI:1.067-4.815,P=0.014),prolactin(OR=4.669,95％CI:1.362-6.251,P＜0.001),and miR-363-3p(OR=4.600,95％CI:2.521-8.325,P＜0.001)were the influencing factors of pregnancy failure after ovulation induction therapy in PCOS patients.Conclusion The relative expression of serum miR-363-3p decreases in PCOS patients,which is correlated with abnormal sex hormone level and glucose metabolic disorder,and PCOS patients with low expression of miR-363-3p have a high risk of pregnancy failure after ovulation induction therapy.
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