The effects of lncRNA GAS5 on the proliferation,invasion ability and PTEN/Akt/mTOR signaling pathway of cervical canc-er cells
Objective This objective of this study was to investigate the effects of long chain non-coding RNA GAS5(ln-cRNA GAS5)on the proliferation,invasion ability and PTEN/Akt/mTOR signaling pathway of cervical cancer cells,and to elucidate the possible mechanism of lncRNA GAS5 in cervical cancer.Methods Real-time quantitative reverse transcription polymerase chain reaction(qRT-PCR)was used to detect the levels of lncRNA GAS5 in human cervical cancer HeLa cells,C33A cells,Caski cells,Siha cells and normal cervical epithelial End1/E6E7 cells.HeLa cells were divided into the blank control group(BC group),negative con-trol group(NC group)and overexpressing lncRNA GAS5 group(GAS5 group);Siha cells were divided into the blank control group(BC group),negative control siRNA group(si-NC group)and siRNA-lncRNA GAS5 group(si-GAS5).The CCK8 assay was used to measure proliferation of cervical cancer cells.The Transwell was used to measure the invasion and migration capabilities of cervical cancer cells.Western blot was used to determine the expression of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase(MMP)-2,MMP-9,PTEN,Akt,phosphorylated Akt(p-Akt),mTOR and p-mTOR proteins in cervical cancer cells.Results The results showed that the levels of lncRNA GAS5 in HeLa cells,C33A cells,Caski cells and Siha cells were lower than that in End1/E6E7 cells(P＜0.001).Compared with the NC group,overexpression of lncRNA GAS5 in HeLa cells resulted in a decrease in the cell proliferation,invasion,and migration abilities,as well as a decrease in the levels of PCNA,MMP-2,MMP-9,p-Akt,and p-mTOR proteins(P＜0.001),while an increase in the level of PTEN protein(P＜0.001).Compared with the si-NC group,inhibition of ln-cRNA GAS5 expression in Siha cells resulted in increased the cell proliferation,invasion,and migration abilities,as well as increased the levels of PCNA,MMP-2,MMP-9,p-Akt,and p-mTOR proteins(P＜0.001),while decreased the level of PTEN protein(P＜0.001).Conclusion The level of lncRNA GAS5 in cervical cancer cells is reduced,and lncRNA GAS5 can inhibit the proliferation,invasion and migration abilities of cervical cancer cells,as well as activate the PTEN/Akt/mTOR signaling pathway.
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