食管鳞癌中microRNA let-7a-3甲基化与IGF-Ⅱ的相关性
The correlation between microRNA let-7a-3 methylation and IGF-Ⅱ expression in esophageal squamous cell carcinoma
朱公建 1郭红云 1白悦 1王晓敏 2朱小康 1刘东彦 1刘玉琴1
作者信息
- 1. 甘肃省医学科学研究院/中山大学附属肿瘤医院甘肃医院科教科(兰州 730050)
- 2. 兰州市西固区人民医院消化内科
- 折叠
摘要
目的 探讨食管鳞癌组织中microRNA let-7a-3 甲基化状态与血浆中类胰岛素样生长因子 2(Insulin like growth factor 2,IGF-Ⅱ)表达的相关性.方法 采用甲基化特异性PCR法(Methylation specific PCR,qMSP)检测83 例食管癌及相对应的癌旁正常组织中let-7a-3 甲基化状态,采用酶联免疫吸附试验(Enzyme linked immunosorbent assay,ELISA)检测血浆中IGF-Ⅱ的表达水平.结果 83 例食管鳞癌患者癌组织中的microRNA let-7a-3 甲基化程度显著高于癌旁正常组织(P<0.001).83 例食管鳞癌患者血浆中IGF-Ⅱ的表达水平与let-7a-3 基因的甲基化程度总体上呈正相关,具有统计学意义(r=0.600,P<0.001).结论 microRNA let-7a-3 可能通过对下游分子的甲基化调控参与食管鳞癌的发生发展,这对了解食管鳞癌形成的机制具有重要意义,可为食管鳞癌的诊断和预后提供依据.
Abstract
Objective The aim of this study was to explore the correlation between the methylation status of microRNA let-7a-3 in esophageal squamous cell carcinoma(ESCC)and the expression of insulin-like growth factor 2(IGF-Ⅱ).Methods The methylation specific PCR(qMSP)was used to detect the methylation status of let-7a-3 in 83 cases of esophageal cancer and corre-sponding adjacent normal tissues.The enzyme linked immunosorbent assay(ELISA)was used to detect the expression of IGF-Ⅱ in plasma.Results The degree of let-7a-3 methylation in cancer tissues of 83 patients with ESCC was significantly higher than that in normal tissues adjacent to cancer(P<0.001).The expression of IGF-Ⅱ in the plasma of 83 patients with ESCC was positively corre-lated with the methylation degree of let-7a-3,which was statistically significant(r=0.600,P<0.001).Conclusion microRNAlet-7a-3 may participate in the occurrence and progression of ESCC by regulating the methylation of downstream molecules,which is of great significance for understanding the mechanisms of ESCC development and providing a basis for the diagnosis and prognosis of ESCC.
关键词
食管鳞癌/let-7a-3/甲基化/类胰岛素样生长因子2Key words
Esophageal squamous cell carcinoma/let-7a-3/Methylation/Insulin like growth factor 2引用本文复制引用
基金项目
兰州市人才创业创新项目(2018-RC-96)
出版年
2024
NETL
NSTL
万方数据