Expression and clinical significance of hypermethylated in cancer 1 in non-small cell lung cancer
Objective The aim of this study was to investigate the correlation between the expression of hypermethylated in cancer 1(HIC1)and overall survival(OS)in non-small cell lung cancer(NSCLC)patients.Methods A total of 2,166 patients with NSCLC,including 1161 patients with adenocarcinoma and 780 patients with squamous cell carcinoma,were collected from the Kaplan-Meier plotter plus(KM plotter)database with detailed pathological type,clinical stage,treatment information,and complete RNA-seq or Gene chip data.Univariate Cox proportional risk regression analysis was performed using KM plotter online database.Multivariate Cox regression analysis corrected for the following confounding factors:gender,smoking history,clinical stage,surgical margin status,chemotherapy and radiotherapy.The relationship between the expression of HIC1 mRNA and OS in 2,166 NSCLC patients was ana-lyzed.Transwell migration assay was used to detect the effect of knockdown HIC1 gene on the migration ability of PC9 cells(lung ade-nocarcinoma cell line).Results In lung adenocarcinoma patients receiving chemotherapy,the high expression of HIC1 significantly prolonged the OS(HR=0.54,95%CI:0.34-0.86,P=0.009),and reduced the risk of death by 46%.Transwell cell migration assay showed that the migration ability in PC9 cells in the low expression of HIC1 was increased(P<0.001).Conclusion The expression of HIC1 mRNA may be an important prognostic predictor for NSCLC patients receiving chemotherapy.
Non-small cell lung cancerHypermethylated in cancer 1Overall survival
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