Expression of transporter associated with antigen processing 1 in gastric adenocarcinoma and its clinical significance
Objective To explore the relationship between the expression of transporter associated with antigen processing 1(TAP1)in stomach adenocarcinoma(STAD)and the clinicopathological characteristics,and its prognostic significance.Methods The clinical data of STAD patients and the TAP1 mRNA expression in the STAD tissues were collected from The Cancer Genome Atlas(TCGA)database.The relationship between TAP1 expression and clinicopathological features was analyzed using GraphPad Prism,while Kaplan-Meier curves were employed to analyze the correlation of TAP1 expression with overall survival.Western blotting was utilized to detect TAP1 protein expression in STAD cell lines,MKN45 and AGS,and human normal gastric mucosal epithelial cell line,GES-1.Additionally,122 STAD tissue specimens and 24 normal gastric resection specimens were collected from the Department of Pathology,Wuhan University Zhong-nan Hospital,between January 1st and December 31st,2020,and between October 1st and December 31st,2020,respectively.The TAP1 expression in these tissues were detected by immunohistochemistry,and its relationship with the clinicopathological features of the patients were analyzed.Results Analysis of TCGA database and immunohistochemistry showed that the TAP1 mRNA and protein expressions in STAD tissues were both significantly higher than those in normal gastric tissues(both P＜0.05).Compared with normal gastric mucosal ep-ithelial cell line GES-1,TAP1 expression was higher in STAD cell line MKN45(P＜0.01),while no significant difference was observed in AGS cells(P＞0.05).TAP1 protein expression in STAD tissues was significantly different in terms of Laurén classification(P＜0.05).TCGA database analysis revealed that the expression of TAP1 increased with the increase of tumor tissue grade(P＜0.05).The STAD patients in TCGA database were categorized into TAP1 high-and low-expression groups based on the median level of 6.82.Survival analysis demon-strated a longer median overall survival in the TAP1 high-expression group(57.1 months vs 25.1 months,P＜0.05).Conclusions TAP1 is highly expressed in STAD tissues and is associated with Laurén classification and tissue grading.It serves as a favorable prognostic factor and may potentially function as a prognostic biomarker for STAD.
stomach adenocarcinomatransporter associated with antigen processing 1tumor immunityantigen presenting
万方数据
维普
