Impact of tertiary lymphoid structures on prognosis and tumor immune microenvironment in melanoma
Objective To investigate the impact of intratumoral tertiary lymphoid structures(TLSs)on the prognosis and tumor immune microenvironment(TIME)in melanoma.Methods A retrospective analysis was conducted on 78 patients with pathologically confirmed melanoma who underwent primary lesion surgical resection between February 2013 and December 2021 at Nanjing Drum Tower Hospital,Affiliated Hospital of Medical School,Nanjing University.HE staining and immunohistochemistry were used to observe TLS characteristics and tumor immune cell infiltration,and to analyze the impact of TLSs on prognosis and TIME.Results Of the 78 patients,38 had intra-tumoral TLSs,while 40 did not.Kaplan-Meier survival analysis suggested that patients with intratumoral TLSs had significantly improved mean overall survival time compared to those without(53.07 months vs 40.69 months,P=0.019).Multivariate Cox regression analysis iden-tified intratumoral TLSs as an independent risk factor affecting the prognosis of melanoma patients(P=0.011).Tissue microarray immuno-histochemistry revealed a higher number of CD3,CD4,CD8,and Foxp3 positive cells per square millimeters in patients with intratumoral TLSs compared to those without(all P<0.05).Conclusions The presence of intratumoral TLSs in melanoma patients was associated with a favorable prognosis and increased immune cell infiltration in TIME.
国家科技期刊平台
NSTL
万方数据
