首页|宫颈液基细胞P16、Ki-67双染检测在1250例宫颈异常患者诊断中的诊断价值

宫颈液基细胞P16、Ki-67双染检测在1250例宫颈异常患者诊断中的诊断价值

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目的:探讨与分析宫颈液基细胞P16、Ki-67 双染检测在 1 250 例宫颈异常患者诊断中的诊断价值.方法:选取 2020 年 1 月至 2023 年 8 月在河南省周口市中心医院进行宫颈疾病筛查的宫颈异常人群 1 250 例作为研究对象,所有患者都给予宫颈液基细胞P16、Ki-67 单染与双染检测,同时给予病理检查,根据病理学检测结果分为宫颈癌组(150 例)和非宫颈癌组(1 100 例).以病理检查作为诊断的金标准,判断以上检测方法对宫颈癌的诊断价值.结果:在 1 250 例患者中,病理检查为宫颈上皮内瘤变(CIN)1 级 600 例、CIN 2 级 320 例、CIN 3 级 180 例及宫颈癌 150例,其中宫颈癌占比 12.00%.宫颈癌组宫颈液基细胞P16、Ki-67 单染阳性与P16、Ki-67 双染阳性分别为 98.00%、98.67%、97.33%,非宫颈癌组分别为 40.55%、49.27%、21.00%,两组对比,差异有统计学意义(P＜0.05).在 1 250 例患者中,宫颈液基细胞P16、Ki-67 单染阳性与P16、Ki-67 双染阳性对宫颈癌的诊断敏感性分别为 98.00%(147/150)、98.67%(148/150)、97.33%(146/150),诊断特异性分别为 59.45%(654/1 100)、50.73%(558/1 100)、79.00%(869/1 100).结论:宫颈异常患者中宫颈癌的发病率比较高,其宫颈液基细胞P16、Ki-67 双染多表现为阳性状况,宫颈液基细胞P16、Ki-67 双染检测在宫颈癌患者诊断中的应用可在保持敏感性的基础上,提高诊断特异性.
Diagnostic Value of P16 and Ki-67 Double Staining Detection in Cervical Fluid Based Cells in 1 250 Patients with Cervical Abnormalities
Objective:To explore and analyze the diagnostic value of P16 and Ki-67 double staining detection in cervical fluid based cells in 1 250 patients with cervical abnormalities.Methods:From January 2020 to August 2023,1 250 cases of individuals with cervical abnormalities who underwent cervical disease screening at Zhoukou Central Hospital were selected as the research subjects.All patients were subjected to single and double staining tests for P16 and Ki-67 in cervical fluid based cells.According to the pathological test results,they were divided into cervical cancer group(150 patients)and non-cervical cancer group(1 100 patients).Using pathological examination as the gold standard for diagnosis,evaluated the diagnostic value of the above testing methods for cervical cancer.Results:The pathological examination showed there were 600 cases of cervical intraepithelial neoplasia(CIN)grade 1,320 cases of CIN grade 2,180 cases of CIN grade 3,and 150 cases of cervical cancer in the 1 250 cases that the cervical cancer accounted for 12.00%.The positive rates of P16 and Ki-67 single staining and P16 and Ki-67 double staining in cervical cancer group were 98.00%,98.67%,and 97.33%,respectively.The rates in the non-cervical cancer group were 40.55%,49.27%,and 21.00%,respectively.There were significant difference between the two groups(P＜0.05).The diagnostic sensitivity of P16 and Ki-67 single staining positive and P16 and Ki-67 double staining positive cervical fluid based cells for cervical cancer were 98.00%(147/150),98.67%(148/150),and 97.33%(146/150),respectively,with diagnostic specificity of 59.45%(654/1 100),50.73%(558/1 100),and 79.00%(869/1 100).Conclusion:The incidence rate of cervical cancer in patients with cervical abnormalities is relatively high,and the double staining of P16 and Ki-67 in the cervical liquid based cells is mostly positive.The application of double staining of P16 and Ki-67 in the diagnosis of cervical cancer patients can improve the specificity of diagnosis on the basis of maintaining sensitivity.

Cervical abnormalitiesCervical cancerCervical fluid based cellsP16Ki-67Double staining

田岩岩、王佳佳

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河南省周口市中心医院 周口 466000

河南省郑州人民医院 郑州 453000

宫颈异常 宫颈癌 宫颈液基细胞 P16 Ki-67 双染

2024

10.13638/j.issn.1671-4040.2024.03.021

实用中西医结合临床
江西省中医药研究院,江西省中西医结合学会

实用中西医结合临床

影响因子：0.522
ISSN：1671-4040
年,卷(期)：2024.24(3)
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