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葛根渣中资源性化学成分分析及膳食纤维提取工艺研究

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目的 对葛根渣中资源性化学成分进行分析,并考察膳食纤维提取最佳工艺,以期为发现葛根渣资源价值和提升资源化利用效率提供参考.方法 通过苯酚-硫酸法、酸碱消煮法分别对葛根渣中的多糖和粗纤维含量进行测定分析,基于单因素试验采用α-淀粉酶、糖化酶和木瓜蛋白酶进行膳食纤维的制备,并测定膳食纤维的膨胀力,确定最佳工艺参数.结果 葛根渣中多糖含量为 10.30%,粗纤维含量为 15.58%;酶法制备膳食纤维的最佳工艺条件为:复合酶(α-淀粉酶、糖化酶)添加比例 1:3、添加总量 4%、酶解时间 75 min、酶解温度 65℃,蛋白酶添加总量为 0.3%.结论 葛根渣中多糖、粗纤维等资源性化学成分丰富,所建立的膳食纤维提取工艺可行,研究结果可为葛根渣的转化增效及粗细高值化利用模式提供科学依据.
Analysis of Resource Chemical Components and Extraction Technology of Dietary Fiber in Pueraria Root Residue
Objective Analyze the resource based chemical components in pueraria root residue and examine the optimal process for extracting dietary fiber,in order to provide reference for discovering the value of pueraria root residue resources and improving resource utilization efficiency.Methods The content of polysaccharides and crude fibers in pueraria root residue was determined and analyzed by using the phenol sulfuric acid method and the acid-base digestion method,respectively.In addition,based on a single factor experiment with α-amylase,saccharifying enzyme,and papain were used to prepare dietary fiber,and the swelling force of dietary fiber was measured to determine the optimal process parameters.Results The polysaccharide content in pueraria root residue was 10.30%,and the crude fiber content was 15.58%.The optimal process conditions for enzymatic preparation of dietary fiber were as follows the proportion of composite enzymes(α-amylase,saccharifying enzyme)added was 1:3,the total amount added was 4%,the enzymatic hydrolysis time was 75 minutes,the enzymatic hydrolysis temperature was 65℃,and the total amount of protease added was 0.3%.Conclusion The resource based chemical components such as polysaccharides and crude fibers in pueraria root residue are abundant,and the established dietary fiber extraction process is feasible.The research results can provide scientific basis for the transformation efficiency enhancement and high-value utilization mode of pueraria root residue.

Pueraria root residueChemical compositionDietary fiberResource value

曾慧婷、郑亚倩、戴迪、何小群、陈超、王小青、虞金宝、李晶

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江西省中医药研究院 南昌 330046

江西省中药资源保护与开发利用工程研究中心 南昌 330046

江西中医药大学 南昌 330004

中药材种质选育繁育重点研究室 江西南昌 330046

江西省中药原料质量监测技术服务中心 南昌 330046

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葛根渣 化学成分 膳食纤维 资源价值

江西省中医药局科技项目江西省卫生健康委科技项目现代农业产业技术体系建设专项

2020B0401202311189CARS-21

2024

实用中西医结合临床
江西省中医药研究院,江西省中西医结合学会

实用中西医结合临床

影响因子:0.522
ISSN:1671-4040
年,卷(期):2024.24(5)
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