Exploring the Mechanism of Seabuckthorn in Preventing and Treating Hypertension Based on Network Pharmacology,Molecular Docking and Antioxidation in Vitro
Objective To explore the main pharmacological components and mechanisms of action of seabuckthorn in the preventing and treating of hypertension by using network pharmacology,molecular docking,and in vitro antioxidant tests,and to provide theoretical reference for further exploring the material basis of seabuckthorn in preventing and treating hypertension.Methods Taked traditional chinese medicine seabuckthorn and hypertension as research objects,active ingredients and targets of drugs were screened through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)database,disease targets were obtained through Gene Cards,NCBI,OMIM,and Uniprot databases,and active ingredient-target-disease networks were constructed using Cystoscape 3.8.0 software.Drawed protein-protein interaction(PPI)networks for targets using String database,and key targets were analyzed for Gene ontology(GO)and gene function analysis(KEGG)using R3.6.3 software.After molecular docking with the active ingredients of seabuckthorn through screening potential targets with high degree values,in vitro antioxidant experiments were conducted on different seabuckthorn extracts to verify.Results A total of 18 active compounds were obtained.The key genes related to diseases were interleukin-6(IL-6),albumin(ALB),serine/threonine kinase(AKT1),vascular endothelial growth factor A(VEGFA),etc.Their functions were mostly related to apoptosis,inflammatory factors,lipid metabolism,cell growth,and induction of oxidative stress;GO and KEGG analysis mainly involve inducing oxidative stress reactions,sodium excretion,etc.Molecular docking showed that all four targets of IL-6,ALB,AKT1,and VEGFA could spontaneously combine with the active ingredients to form stable conformations.In vitro antioxidant experiments have shown that seabuckthorn extracts rich in vitamin C,sterols,and flavonoids have strong free radical scavenging and reducing abilities.Conclusion Seabuckthorn has a potential mechanism of action with multiple components,multiple targets,and multiple pathways in preventing and treating hypertension.Its extracts rich in related active compounds have high free radical scavenging and reducing abilities,which may regulate the expression of related protein targets such as IL-6,ALB,AKT1,VEGFA,and regulate related signaling pathways,thereby playing a role in reducing blood lipids and regulating blood pressure.
万方数据
维普
