Preparation and titer determination of specific antibody of Agkistrodon halys
Objective The preparation of specific antibodies to Agkistrodon halys,provided theoretical basis for further research and development of antivenoms.Methods Using the venom protein of Agkistrodon halys as antigen,the specific antibody of Agkistrodon halys was prepared by immunizing New Zealand rabbits.Its titer was detected by indirect ELISA and its specificity was detected by Western blot.Results The specific antibody serum of Agkistrodon halys was successfully prepared,and its titer was 1∶ 100000.Western blot analysis showed that the antibody was specific to Agkistrodon halys venom.Conclusion This study successfully pre-pared the specific antibody of Agkistrodon halys and promoted the research and development of anti-Agkistrodon halys venom serum.
NETL
NSTL
万方数据
维普
