蝮蛇特异性抗体的制备及其效价测定
Preparation and titer determination of specific antibody of Agkistrodon halys
朱俊蓉 1陆雪玲 1黄周 2黄东凌 2王璠 2韦晚霞 2刘恋 1黄永龙 1王威1
作者信息
- 1. 广西医科大学第一附属医院急诊科,广西南宁 530021
- 2. 广西高校急诊医学重点实验室,广西南宁 530021
- 折叠
摘要
目的 制备蝮蛇特异性抗体,为进一步研发抗蛇毒血清提供理论依据.方法 以蝮蛇蛇毒蛋白作为抗原,通过免疫新西兰兔制备蝮蛇特异性抗体,应用间接ELISA法检测其效价,Western blot检测其特异性.结果 成功制备了蝮蛇特异性抗体血清,其效价可达1∶ 100000,Western blot检测抗体对蝮蛇蛇毒具有专一性.结论 本研究成功制备了蝮蛇特异性抗体,推进了抗蝮蛇毒血清的研发.
Abstract
Objective The preparation of specific antibodies to Agkistrodon halys,provided theoretical basis for further research and development of antivenoms.Methods Using the venom protein of Agkistrodon halys as antigen,the specific antibody of Agkistrodon halys was prepared by immunizing New Zealand rabbits.Its titer was detected by indirect ELISA and its specificity was detected by Western blot.Results The specific antibody serum of Agkistrodon halys was successfully prepared,and its titer was 1∶ 100000.Western blot analysis showed that the antibody was specific to Agkistrodon halys venom.Conclusion This study successfully pre-pared the specific antibody of Agkistrodon halys and promoted the research and development of anti-Agkistrodon halys venom serum.
关键词
蝮蛇蛇毒/抗体制备/效价测定Key words
Agkistrodon halys venom/Antibody preparation/Titer determination引用本文复制引用
基金项目
区域创新合作项目(2022YFQ0085)
出版年
2024
NETL
NSTL
维普
万方数据