Screening & Identification and Characteristics Study of the Sulfadiazine Degrading Bacteria
Taking the sulfadiazine as the target removal material,an efficient degradation strain Y23 with the sole carbon and nitrogen source was separated from acclimated activated sludge of a wastewater treatment plant.Morpho-logical observation,physiological and biochemical assays,16S rRNA gene sequencing,and phylogenetic analysis were conducted to identify the strain.The bacterial population and the degradation rate of sulfadiazine by strain Y23 were analyzed under different environmental conditions.High-performance liquid chromatography-tandem mass spectrometry(LC/MS)was employed to determine the intermediate products of sulfadiazine degradation and eluci-date the degradation pathways.The findings indicated that Bacillus cereus was the proficient decomposer;The most favorable degradation conditions of strain Y23 were an initial pH value of 7,a temperature setting of 30℃,a stir-ring rate of 150 r/min,and an inoculation volume of 5.0％.After 72 hours of incubation,the degradation efficiency reached 81.40％;A total of 16 intermediate products were detected by LC/MS,and four degradation pathways were proposed,involving oxidation,hydrolytic ring-opening,decarboxylation,and deamination processes.The findings provide a microbial basis for the efficient treatment of sulfadiazine-contaminated wastewater in engineering applica-tions.
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