Application of DNA Strand Displacement Reaction in the Visual Keypad Lock Security System
In this paper,three modules are designed by using DNA strand replacement reaction:input module,reac-tion module and detection module.The input module can add single strands in different sequences to the reaction module.Only the DNA strand with the correct input sequence can preferentially undergo a strand displacement reac-tion with the combined substrate of the reaction module to replace a single strand of DNA.Two types of strand dis-placement reactions were designed for the detection module,and threshold comparison was used to determine the number of times the correct sequence of DNA strands was inputted.This study uses fluorescence detection to deter-mine whether unlocking is successful,achieving visualization of the results.The system adopts a modular structure,and the detection method is simple and intuitive.Only when the input DNA strand order and frequency are correct,can the keyboard lock be accurately unlocked,increasing the key space and having excellent stability and security,providing the possibility of building more complex information security systems.
DNA strand displacementVisual keypad lockLogic gateThreshold comparison
万方数据
维普
