人卵泡液中肾素-血管紧张素系统与年龄、卵巢功能及IVF实验室结局的相关性研究
Association of the renin-angiotensin system components in human follicular fluid with age,ovarian function and IVF laboratory outcomes
魏仑 1骆超 1伯乐 1周安稳 1吴志南 1陆宣平 1占雷 1高莎莎 1钱菲 1茅彩萍1
作者信息
- 1. 苏州大学附属第一医院生殖医学中心,苏州 215006
- 折叠
摘要
目的 探索人卵泡液(human follicular fluid,hFF)中肾素-血管紧张素系统(renin-angiotensin system,RAS)与年龄、卵巢功能及体外受精(in vitro fertilization,IVF)实验室结局的相关关系.方法 设计非干预的观察性研究,收集2021年1月至2022年2月期间在苏州大学附属第一医院生殖医学中心单纯因男方因素接受IVF女性患者的hFF及不包含个人身份的病历信息.采用酶联免疫吸附试验试剂盒检测hFF中的肾素、血管紧张素转换酶(angiotensin converting enzyme,ACE)、ACE2、血管紧张素(angiotensin,Ang)Ⅱ和Ang 1-7的水平.一元线性回归用于RAS组分与年龄的相关性分析,多元线性回归用于RAS组分与卵巢功能及IVF实验室结局的相关性分析.结果 ①共获139例可分析样本.②年龄与肾素(Pearson's r=-0.313 3,P＜0.001)、ACE(Pearson's r=-0.183 6,P= 0.031)、AngⅡ(Pearson's r=-0.218 6,P=0.010)、ACE/ACE2(Pearson's r=-0.319 2,P＜0.001)、AngⅡ/Ang1-7(Pearson's r=-0.224 3,P=0.008)存在线性负相关关系,而与ACE2、Ang1-7的线性关系不显著(均P＞0.05).③基础卵泡刺激素与年龄(β=0.636,P＜0.001)、ACE2(β=0.267,P=0.026)和AngⅡ(β=0.268,P= 0.001)呈正相关,与ACE(β=-0.320,P＜0.001)和Ang1-7(β=-0.217,P=0.014)呈负相关;黄体生成素与AngⅡ(β=0.330,P=0.003)呈正相关,与Ang1-7(β=-0.395,P=0.002)呈负相关;窦卵泡计数与Ang1-7(β= 0.153,P=0.049)呈正相关,与年龄(β=-0.869,P＜0.001)和ACE2(β=-0.082,P=0.004)呈负相关;基础抗苗勒管激素仅与年龄(β=-0.349,P＜0.001)呈负相关;基础雌二醇、孕酮、泌乳素、睾酮与年龄及RAS的相关关系不显著(均P＞0.05).④获卵数与肾素(β=0.146,P=0.014)、AngⅡ(β=0.113,P=0.034)和Ang1-7(β=0.185,P=0.002)呈正相关,与年龄(β=-0.717,P＜0.001)呈负相关;MⅡ卵成熟率与AngⅡ(β=0.207,P=0.019)和Ang1-7(β=0.217,P=0.026)呈正相关,与年龄(β=-0.518,P＜0.001)呈负相关;双原核胚胎率、可移植胚胎率、优质胚胎率仅与年龄呈负相关(均P＜0.05).结论 卵泡局部RAS受到年龄影响,且与卵巢功能及IVF实验室结局存在一定关系.
Abstract
Objective To investigate the association between the local renin-angiotensin system(RAS)in human follicular fluid(hFF)and age,ovarian function,in vitro fertilization(IVF)laboratory outcome.Methods A non-intervention observational study was designed.hFF and medical case history without personal identity of patients who received IVF simply because of male factor infertility in Reproductive Medicine Center,the First Affiliated Hospital of Soochow University during January 2021 and February 2022 were collected.The renin,angiotensin converting enzyme(ACE),ACE2,angiotensin(Ang)Ⅱ and Ang 1-7 levels were detected by enzyme-linked immunosorbent assay kit.The correlation between age and RAS in hFF was analyzed by simple linear regression,and multivariate linear regression was used to further analyze the correlation between the RAS and IVF laboratory outcome.Results 1)A total of 139 samples of analysable hFF were obtained.2)There was a linear negative correlation between age and renin(Pearson's r=-0.313 3,P＜0.001),angiotensin converting enzyme(ACE;Pearson's r=-0.183 6,P=0.031),angiotensinⅡ(AngⅡ;Pearson's r=-0.218 6,P=0.010),ACE/ACE2(Pearson's r=-0.319 2,P＜0.001),Ang Ⅱ/Ang1-7(Pearson's r=-0.224 3,P=0.008),while the linear relationship with ACE2 and Ang1-7 was not significant(all P＞0.05).3)Basal follicle-stimulating hormone was positively correlated with age(β= 0.636,P＜0.001),ACE2(β=0.267,P=0.026)and Ang Ⅱ(β=0.268,P=0.001),while negatively correlated with ACE(β=-0.320,P＜0.001)and Ang1-7(β=-0.217,P=0.014).Basal luteinizing hormone was positively correlated with AngⅡ(β=0.330,P=0.003),while negative correlated with Ang1-7(β=-0.395,P=0.002).Antral follicle count was positively correlated with Ang1-7(β=0.153,P= 0.049),while negatively correlated with age(β=-0.869,P＜0.001)and ACE2(β=-0.082,P=0.004).Basal anti-Müllerian hormone was only negatively correlated with age(β=-0.349,P＜0.001).There was no correlation between RAS and basal estradiol,progesterone,prolactin,testosterone(all P＞0.05).4)Oocyte retrieval count was positively correlated with renin(β=0.146,P=0.014),AngⅡ(β= 0.113,P=0.034)and Ang1-7(β=0.185,P=0.002),while negatively correlated with age(β=-0.717,P＜0.001);MⅡ oocyte maturation rate was positively correlated with AngⅡ(β=0.207,P=0.019)and Ang1-7(β=0.217,P=0.026),while negatively correlated with age(β=-0.518,P＜0.001).There was no correlation between RAS and the rates of two pronuclei embryos,transplantable embryos,high-quality embryos(all P＞0.05).Conclusion The local RAS in ovarian follicles is affected by age and correlated with ovarian function and IVF laboratory outcome.
关键词
受精,体外/卵泡液/肾素-血管紧张素系统/年龄/卵巢Key words
Fertilization in vitro/Follicular fluid/Renin-angiotensin system/Age/Ovary引用本文复制引用
基金项目
国家自然科学基金(81671535)
国家科技支撑计划项目(2013BAI04B05)
江苏省人类辅助生殖医学基金重点学科(FXK202149)
江苏省卫健委医学基金重点学科(ZDB2020007)
苏州市重大项目研究(20220901)
出版年
2024
NETL
NSTL
万方数据