Influence of morphological evaluation parameters of blastocysts on chromosomal karyotype abnormalities of chorionic villi in missed abortion after IVF/ICSI treatment
Objective Analyzing the influence of morphological evaluation parameters of blastocysts,including days of blastocyst development[day 5(D5)and day 6(D6)],degree of blastocyst expansion(4,5,6),inner cell mass and trophectoderm grade,on the occurrence of chromosomal karyotype abnormalities of chorionic villi in missed abortion after in vitro fertilization/intracytoplasmic sperm injection(IVF/ICSI)treatment and fresh/frozen-thawed single blastocyst transfer.Methods The clinical data of patients with missed abortion after IVF/ICSI treatment and fresh/frozen-thawed single blastocyst transfer from February 2015 to February 2023 in the Reproductive Center of the Third Affiliated Hospital of Zhengzhou University were included.Using a case-control study,the data were divided into two groups according to the detection results of chromosomal copy number variations(CNVs)in chorionic villi of missed abortion abnormal karyotype group(n=139)and normal karyotype group(n=82).The baseline data between the two groups were compared.Univariate logistic regression was used to investigate the effect of blastocyst morphological rating parameters on the occurrence of chromosomal karyotype abnormalities of chorionic villi in aborted tissues,and multivariate logistic regression was also used to adjust confounding factors.Results Male age[(34.12±6.49)years],sperm morphology rate[5.00(4.00,6.00)％]and female age[33.00(30.00,37.00)years]in abnormal karyotype group were higher than those in the normal karyotype group[(32.38±4.69)years,4.00(2.00,5.00)％and 31.50(29.00,34.00)years],and the differences were statistically significant(P=0.022,P=0.020,P=0.009).Univariate and multivariate logistic regression analyses showed that days of blastocyst development,degree of blastocyst expansion,inner cell mass and trophectoderm grade did not increase the risk of chromosomal karyotype abnormalities of chorionic villi(all P＞0.05).Conclusion There is no significant correlation between blastocyst morphological evaluation parameters and chromosomal karyotype abnormalities in chorionic villi of missed abortion after fresh/frozen-thawed single blastocyst transfer with IVF/ICSI treatment.
Single blastocystEmbryo morphological parametersInner cell massTrophectodermMissed abortionChromosome of chorionic villi
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