从受精卵到囊胚期胚胎在体外培养发育过程中的果糖代谢
Fructose metabolism from zygote to blastocyst stage development during culture in vitro
魏士喜 1千日成1
作者信息
- 1. 同济大学附属第十人民医院生殖医学中心,上海 200072
- 折叠
摘要
哺乳动物胚胎在早期发育阶段是以丙酮酸和乳酸为主要代谢途径产生ATP,但是随着胚胎基因组的激活和生物合成的增加,其能量代谢也会发生动态变化,随着胚胎发育到囊胚阶段,能量来源逐步转变为葡萄糖代谢.早期研究表明,培养液中的葡萄糖会抑制早期胚胎发育.动物研究显示,果糖可以代替葡萄糖作为能量基质被胚胎摄取利用.培养液中添加果糖可以提高动物胚胎体外培养的囊胚形成率.培养液中混合添加果糖与葡萄糖还可以改善动物囊胚质量、降低细胞凋亡比例.由此推测,果糖和葡萄糖在培养液中具有一定的代谢协同作用可改善胚胎的发育.本文通过分析讨论受精卵到囊胚期胚胎在体外培养发育过程中的能量底物需求,从而对胚胎培养液中添加果糖可能的代谢途径进行概述.
Abstract
Dynamic changes of energic metabolism occur in mammalian embryonic development from zygote to blastocyst stage.Pyruvate and lactate play an important role in early cleavage stage for ATP production,and glucose may play a key role for blastocyst development after zygote genome activation.Although the early cleavage development is inhibited by glucose,the late blastocyst formation is required for existing glucose in the culture medium in vitro.It has been reported that the role of glucose in the culture medium can be replaced by fructose.In animal studies,it also has been reported that the blastocyst formation rate is increased by fructose supplementation in the culture medium,and further indicated that combined use of glucose and fructose in the culture medium as energic sources not only increases the blastocyst formation rate but also improves the quality of blastocyst and reduces the cell apoptosis.Those results indicated that there is synergic function of glucose and fructose in the culture medium during embryonic development.In this review,we will discuss the possibility of fructose metabolic routes from the development of zygote to blastocyst stage and explain the functional role of fructose in the culture medium.
关键词
受精卵/囊胚/胚胎发育/果糖/能量代谢Key words
Zygote/Blastocyst/Embryo development/Fructose/Energic metabolism引用本文复制引用
出版年
2024
NETL
NSTL
万方数据