表没食子儿茶素没食子酸酯对博莱霉素所致肺纤维化大鼠肺组织中TGF-β1及Smad2/7mRNA表达的影响
Effect of epigallocatechin gallate(EGCG) on the expression of TGF-β1 and Smad2/7mRNA of lung tissue of the experimental lung pulmonary fibrosis in rats
周艳 1顾振纶 1蒋小岗 1马荣林 2蔡健 1郭次仪3
作者信息
- 1. 苏州大学医学部药学院药理学系,江苏苏州215123;苏州中药研究所,江苏苏州215007
- 2. 苏州中药研究所,江苏苏州215007
- 3. 苏州中药研究所,江苏苏州215007;香港保健协会,香港
- 折叠
摘要
目的 观察表没食子儿茶素没食子酸酯(EGCG)对博莱霉素所致大鼠肺纤维化的作用及其机制.方法 健康SD大鼠随机分为正常组、模型组、泼尼松组及EGCG大、中、小剂量组,经气管内滴注博莱霉素复制肺纤维化模型,于造模后第2天开始给药,给药28 d后,取肺组织作病理形态学观察并测定其羟脯氨酸含量,采用RT-PCR法检测肺组织中转化生长因子(TGF)-β1、Smad2、Smad7 mRNA的表达.结果 EGCG各给药组大鼠肺组织肺泡炎和肺纤维化程度明显减轻(P＜0.05或＜0.01),羟脯氨酸含量明显降低(P＜0.05或＜0.01).RT-PCR检测结果显示,EGCG给药组大鼠TGF-β1、Smad2表达下调(P＜0.05或＜0.01),Smad7表达明显上调(P＜0.05或＜0.01).结论 EGCG可减轻博莱霉素所致大鼠肺纤维化程度,其作用机制可能与其调控TGF-β1/Smad信号通路有关.
Abstract
Objective To investigate the mechanisms of epigallocatechin gallate (EGCG)on Bleo-mycin-induce pulmonary fibrosis in rats. Methods Sprague-Dawley rats were randomly divided into control , model, prednisolone group and EGCG large-dose, middle-dose, small-dose group. Pulmonary fibrosis model was established by intratracheal injection of bleomycin (5mg/kg). Then the rats were treated with corresponding dose of EGCG by intragastric administration. After 28 days, the extent of pulmonary fibrosis was evaluated by histopathological analysis. The extent of pulmonary alveolitis and fibrosis were e-valuated by histopathological and biochemical analysis. The level of TGF-β、 Smad2 and Smad7 mRNA were assayed by semi-quantitative RT-PCR. Results EGCG reduced the extent of alveolitis, fibrosis (P <0.05 or <0.01), and dramatically decreased the content of HYP in the lung tissue(P <0. 05 or < 0.01). EGCG also decreased the level of TGF-pi ,Smad2 mRNA( P <0.05 or <0.01) while the expression of Smad7 mRNA in lung tissue of rats was significantly increased (P <0.05 or <0.01). Conclusion EGCG could alleviated bleomycin-induced pulmonary fibrosis in rats, which might be related with inhibition of TGF-p singnal pathway.
关键词
肺纤维化/表没食子儿茶素没食子酸酯/转化生长因子-β1/信号通路Key words
pulmonary fibrosis/epigallocatechin gallate/transforming gowth factor-β1/signaling pathway引用本文复制引用
基金项目
香港保健协会研究项目(HK2010091622)
江苏省中医药管理局资助项目(HZ07088)
苏州市科技局资助项目(SZ08088)
出版年
2012
NETL
NSTL
维普
万方数据