受体酪氨酸激酶Axl调控糖蛋白Ⅵ介导的血小板活化
Receptor tyrosine kinase Axl participates in glycoprotein VI-mediated platelet activation
王璐 1周俊松 1武艺1
作者信息
- 1. 苏州大学医学部唐仲英血液学研究中心,江苏苏州215123
- 折叠
摘要
目的 研究生长静止特异性基因6的受体Axl对胶原蛋白受体糖蛋白Ⅵ介导的血小板活化的调控作用.方法 利用Axl基因敲除小鼠和糖蛋白Ⅵ特异性刺激剂,在体外分别研究血小板的聚集和铺展功能,并应用流式细胞术检测血小板的颗粒释放和整合素αⅡbβ3的活化.结果 与野生型小鼠相比,Axl基因敲除小鼠的血小板聚集和铺展功能受到明显抑制；流式细胞术检测结果表明,Axl敲除的血小板的颗粒释放标记物CD62P的表达明显降低(P＜0.05),整合素αⅡbβ3的活化同样受到明显抑制(P＜0.05).结论 生长静止特异性基因6的受体Axl在糖蛋白Ⅵ介导的血小板活化中发挥重要的调控作用.
Abstract
Objective To determine the role of growth arrest specific gene 6 (Gas6) receptor Axl in glycoprotein VI (GPVI) -mediated platelet activation. Methods We isolated platelets from wild type and Axl knockout ( Axl~ ) mice, and analyzed their responses to GP VI agonist by measuring aggregation, spreading,granule release (P-selectin expression) and integrin a D b|33 activation. Results Compared with wild type platelets, Axl-deficient platelets displayed a decrease in aggregation and spreading on fi-brinogen upon GPVI stimulation(P <0. 05) , and reduced surface expression of P-selectin and suppression of integrin ot D b|33 activation (P < 0.05). Conclusion Gas6 receptor Axl plays an important role in GPVI-mediated platelet activation.
关键词
生长静止特异性基因6/Axl/糖蛋白Ⅵ/血小板活化Key words
growth arrest specific gene 6/Axl/glycoprotein VI/platelet activation引用本文复制引用
基金项目
苏州大学“211工程”建设经费项目(14121903)
出版年
2012
NETL
NSTL
维普
万方数据