辛伐他汀对人肾小管上内皮细胞蛋白C受体表达的影响
The role of Simvastatin on the expression of endothelial protein C receptor on human renal tubular epithelial cells
钱文娟 1卢国元 2褚伟强 3曹永珍 1顾毅峰 1王云1
作者信息
- 1. 张家港市第一人民医院肾内科,江苏张家港215600
- 2. 苏州大学附属第一医院肾内科,江苏苏州215006
- 3. 张家港市第一人民医院检验科,江苏张家港215600
- 折叠
摘要
目的 探讨辛伐他汀对人肾小管上皮细胞(HKC)上内皮细胞蛋白C受体(EPCR)的影响.方法 采用流式细胞仪检测HKC上EPCR的表达,并将肿瘤坏死因子-α(TNF-α)和辛伐他汀作用于HKC,采用RT-PCR技术对上述因子作用后的HKC上EPCR mRNA表达进行检测.结果 EPCR在HKC中有高表达,TNF-α可下调HKC中EPCR mRNA的表达,而辛伐他汀可以明显改善TNF-α对EPCR的抑制.结论 HKC中EPCR的表达可受到TNF-α的影响,辛伐他汀对上述影响因素有一定拮抗作用,对肾小管上皮细胞损伤可能有保护作用.
Abstract
Objective To investigate the effect of Simvastatin on endothelial protein C receptor (EPCR) in Human Renal Tubular Epithelial Cells (HKC). Methods The expresson of EPCR in HKC was detected by flow cytometry treated by tumor necrosis factor alpha (TNF-a) and Simvastatin. The expression of EPCR in HKC was detected by RT-PCR. Results The results showed that the expression of EPCR were significantly increased in HKC, and the level of EPCR in HKC was suppressed by TNF-a. Simvastatin can obviously reverse the inhibition on EPCR of HKC. Conclusion It was suggested that the expression of EPCR in HKC could be suppressed by TNF-a. Simvastatin may have a protective effect on the renal tubular epithelial cell injury by inhibiting the effect of TNF-a on EPCR.
关键词
内皮细胞蛋白C受体/肿瘤坏死因子-α/辛伐他汀Key words
endothelial protein C receptor (EPCR)/tumor necrosis factor alpha (TNF-a)/Simvastatin引用本文复制引用
基金项目
张家港市2010年科技发展计划项目(ZKS1008)
出版年
2012
NETL
NSTL
维普
万方数据