脂多糖预处理对大鼠心肌缺血再灌注损伤的保护作用
The protective effects induced by lipopolysaccharide precondition on myocardial ischemia and reperfusion injury in rats
于昆 1李江津 1田晓沂1
作者信息
- 1. 淮安市第一人民医院心血管内科2科,江苏淮安,223300
- 折叠
摘要
目的 探讨脂多糖(LPS)预处理对大鼠心肌缺血再灌注(L/R)损伤的保护作用及机制.方法 建立大鼠在体心肌I/R模型,24只SD大鼠随机分为3组:假手术组(Sham组),缺血再灌注组(I/R组),LPS预处理组(ET组).HE染色观察各组心肌形态学改变,RT-PCR法检测心肌组织中白细胞介素-1受体相关激酶-4(IRAK-4) mRNA水平,Western-Blot法检测心肌组织IRAK-4蛋白水平,免疫组化方法检测心肌组织中NF-KB的表达,ELISA法检测血清TNF-α含量.结果 HE染色提示ET组心肌细胞损伤较I/R组明显减轻.ET组及I/R组心肌组织IRAK-4mRNA水平及蛋白水平、NF-KB及血清TNF-α含量明显高于Sham组(均P＜0.01),并且ET组的表达水平又明显低于I/R组(P＜0.01).结论 抑制IRAK-4表达进而抑制NF-KB及TNF-α的表达,可能是LPS预处理减轻心肌I/R的重要机制之一.
Abstract
Objective To investigate the protective effects and mechanisms of Lipopolysaccharide (LPS) precondition on myocardial ischemia and reperfusion injury (I/RI) in rats in vivo. Methods 24 Sprague-Dawley rats were divided randomly into 3 groups (n = 8): the ischemia/reperfusion group (I/R group), the Lipopolysaccharide Precondition group(ET group), and the Sham operated group (Sham group) as control group. The morphology of myocardium was analyzed by light microscopy respectively. The expression of IRAK-4 gene and protein level of myocardium was determined by reverse transcription-polymerase chain reaction( RT-PCR) and Western Blot, the activities of NF-kB was detected by immuno-histochemical method and the serum TNF-a level was estimated by enzyme-linked immunosorbent assay ( ELISA). Results The injury of myocardial cell on ultrastructural features in ET group was significantly reduced vs the I/R group. The expression of IRAK-4mRNA and the protein level also the activities of NF-kB and the content of serum TNF-a in ET and IR group were higher than those in the Sham group (P < 0.01), however, all those detected indexes were significantly reduced in ET group compared with those in IR group(P <0.01). Conclusion LPS precondition on myocardial I/RI in rats was identified to has protective effects by inhibiting the expression of IRAK-4, NF-kB and TNF-a subsequently.
关键词
脂多糖/缺血再灌注/白细胞介素-1受体相关激酶-4/心肌Key words
lipopolysaccharide/ischemia and reperfusion injury/interleukin-1 receptor associated引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据