高效液相色谱法测定秋鼠麴草中总黄酮的含量
Determination of the total flavonoid of Gnaphalium affine D.Don by high-performance liquid chromatography
陆叶 1陈桢 2肖旭 1刘春宇1
作者信息
- 1. 苏州大学医学部药学院中药鉴定研究室,江苏苏州215123
- 2. 苏州市常熟食品药品监督管理局,江苏苏州215500
- 折叠
摘要
目的 建立秋鼠麴草提取物中总黄酮含量高效液相色谱的测定方法.方法 选择乙醇浓度、料液比和提取时间这3个因素进行正交试验,初步优选出常规热回流法提取总黄酮的最佳工艺.色谱柱为HypersilODS2 C18 柱(250 mm ×4.6mm,5μm)；流动相为甲醇∶水=33∶67;紫外检测波长为359 nm；流速1.0 ml/min；柱温35℃；以芦丁为对照计算总黄酮含量；进样量10μl.结果 最优的总黄酮提取条件为:60％乙醇溶液、料液比为1∶35、提取时间为60 min.芦丁在0.013 2～0.105 6 mg/ml范围内线性良好,样品平均回收率为98.98％,相对标准偏差(RSD)为1.86％.结论 利用高效液相色谱法测定秋鼠麴草热回流提取物中总黄酮含量的方法回收率高、精密度好、重复性好,能为药材鼠麴草的质量评价和控制提供可行方法.
Abstract
Objective To develop a high-performance liquid chromatography (HPLC) method for determination of the amount of total flavonoid from Gnaphalium hypoleucum DC. Methods The preliminary optimum extracting conditions of total flavonoid were investigated by using orthogonal experimental design. The experimental factor,such as the percentage of ethanol,the ratio of material to liquid, time of the ethanol refluxing technology was studied. HypersilODS2 C18(250 mm x4. 6 mm, ujn) column of (HPLC) was adopted in the flavonoid determination. The MEOH-water (33: 67) was used as mobile phase. The flow rate was 1. 0 ml/min. The temperature of HypersilODS2 Clgwas 35t. The detective wavelength was 359 run. The concentration of Rutin was used as control and the injection volume was 10uX. Results The optimal extracting conditions were as follows; 60% ethanol solution, the ratio of material to liquid was l-'35; the extracting time was 60 min. Rutin in 0.0132 -0.1056mg/ml range was obtained good linearity. The average recovery was 98.98% with the RSD of 1.86%. Conclusion It was found that HPLC method could be used for determination the concentration of total flavonoid of hot reflux extraction of Gnaphalium hypoleucum DC. This method proved to be high recovery, good precision and good repeatability providing a feasible means for quality evaluation and control of Herb Gnaphalii.
关键词
秋鼠麴草/芦丁/高效液相色谱法/总黄酮/含量测定Key words
Gnaphalium hypoleucum DC./Rutin/high-performance liquid chromatography/total flavonoids/determination content引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据