Objective To investigate the effect of Resveratrol combined with X-ray on radiosensitivity in glioma stem cells. Methods The proliferation inhibition of glioma stem cells induced by X-rays and Resveratrol was assessed with MTT assay. The activation of proapoptotic effect was characterized by Ho-echst 33258 stain. MDC stain and Western blot analysis were used to analyze the autophagy mechanism in X-rays-induced death of glioma stem cells. Results MTT assay indicated that X-rays and Resveratrol decreased the viability of glioma stem cells (P <0. 05) ; we found the proliferative inhibition of glioma stem cells was declined when we used 3-MA to inhibit autophagy (P <0. 05). When the cells were treated by the Resveratrol and x-rays, their spherical shape were changed. Apoptosis was induced in glioma stem cells by combined X-rays and Resveratrol as detected by Hoechst 33258 staining. In addition, autophagy was induced in glioma stem cells in the combined treatment group as detected by MDC staining. Western blotting showed that Bcl-2 expression was decreased in the combined treatment group (P <0. 01) , and the LC3-II expression was increased in the combined treatment group (P <0.01). Conclusion Resveratrol can increased the radiation sensitivity of glioma stem cells, the apoptosis and autophagy was induced in the glioma stem cells in the combined treatment X-rays and Resveratrol. Our results suggest that autophagy plays an essential role in the regulation of radiosensitization of glioma stem cells.
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