硫酸镁对新生大鼠神经干细胞辐射损伤的保护作用
The protective effect of magnesium sulfate against irradiation injury of neural stem cells in neonatal rats
黄辉 1刘萍 2陈娜 3涂彧3
作者信息
- 1. 桂林医学院附属医院放疗科,广西桂林541001
- 2. 扬州市苏北医院,江苏扬州225001
- 3. 苏州大学放射医学与防护学院,江苏苏州215123
- 折叠
摘要
目的 研究硫酸镁对电离辐射所致的神经干细胞损伤的保护作用.方法 取新生大鼠神经干细胞进行培养,分为正常对照(空白对照)组、实验对照(单纯照射)组和实验(照射+硫酸镁)组.60Coγ射线照射2Gy后透射电镜下观察神经干细胞的凋亡形态；60Coγ射线分别照射2Gy、4Gy后24h、48 h用流式细胞仪检测细胞周期分布情况.结果 与正常对照组相比,实验对照组的神经干细胞可见明显凋亡,细胞周期出现明显G1期、G2期阻滞(均P＜0.05).实验组的神经干细胞凋亡数量较实验对照组明显减少,神经干细胞的周期阻滞也较实验对照组明显减轻(均P＜0.05).结论 硫酸镁可降低电离辐射对神经干细胞的损伤作用,辐射后的细胞凋亡、细胞周期阻滞情况都有所缓解.
Abstract
Objective To study the protection of magnesium sulfate against radiation-induced injury of neural stem cells. Methods Brain tissues of new-born Sprague-Dawley(SD) rats were dissociated to culture the neural stem cells. The neural stem cells were divided into 3 groups as blank control group, experimental control group and experimental group (with magnesium sulfate). Observe neural stem cell ap-optosis after being irradiated with 2 Gy of gamma rays,detect the cell cycle by FCM on 24h and 48h after being irradiated with 2 Gy,4 Gy. Results Compared with the blank control group, the apoptosis of neural stem cells in the experimental control group was obvious,and the neural stem cells were blocked in G, , G2phase obviously. Compared with the experimental control group,the number of the apoptotic cells in the experimental group decreased and the cell cycle blocking was also reduced significantly ( P < 0. 05). Conclusion Magnesium sulfate can alleviate the injury of neural stem cells; ease the apoptosis and the cell cycle blocking after irradiation.
关键词
神经干细胞/硫酸镁/细胞凋亡/细胞周期阻滞/电离辐射/新生大鼠Key words
neural stem cell/magnesium sulfate/cell apoptosis/block cell cycle/ionizing radiation/neonatal rats引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据