Objective To study the radiation sensitization effect of miRNA-34c on A549 cell. Methods The cells were divided into transfection, transfection-irradiation and irradiotion groups. The trarsfection group was transfected with miRNA-34c series. The transfection-irradiation and irradiation groups were irradiated with or without miRNA-34c transfection respectively. Then cell growth inhibition ratio was measured by MTT, cell cycle was detected by FCM ,cell apoptosis ratio was observed by Erythro-sin B staining and p53 expression was visulized by Western blot. Results The inhibition ratio and apoptosis ratio of the group with transfection-irradiation combined treatment was apparently higher than those treated by transfection or irradiation and rose as the transfection density increases (P <0. 05). The cell cycle analysis showed that the cell was obviously blocked in G1/Go Phase. Compared with the irradiation group, the p53 protein expression of the cell with combined treatment increased in a dose dependent ma-ner. Conclusion The miRNA-34c has the radiation sensitization effect on A549 adenocarcinoma cell, through enhancing the expression of p53 protein and inducing the cell cycle blockade and apoptosis.
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