Biodistribution and radioimmunoimage of iodine-125 labeled anti-human ADAM15 polyclonal antibody in nude mice bearing human gastric carcinoma xenografts
Objective To investigate the biodistribution of Iodine-125 labeled anti-human ADAM 15 polyclonal antibody in nude mice bearing human gastric carcinoma xenografts. Methods The anti-human ADAM15 polyclonal antibody was labeled with 1-125 using Chloramine-T method. The labeling efficiency and radiochemical purity of 125I-anti-ADAM15 antibody were measured. The SPECT plannar imaging of nude mice bearing gastric carcinoma xenografts were performed at 1, 4, 8, 24, and 48h post-injection and the biodistribution of l25I-anti-ADAM15 antibody was measured at 48 h after injection. Results The labeling efficiency of 125I-anti-ADAM15 antibody was (75.16 ±9.43) % and its radiochemical purity was (99.44 ±0.21) %. Tumors could be cleanly visualized in SPECT planar images, and the radioactivity ratio of tumor to non-tumor tissue was 3. 84 ±0. 43 at 48 h post-injection. Conclusion l251-anti-AD-AM15 antibody can target the gastric carcinoma in vivo, and provide good radioimmunoimages.
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