Objective Our project aims to provide reliable evidences that there are estradiol receptors on the membrane of murine bone marrow-derived macrophages ( BMMs) , which will break through the traditional idea of estadiol action and provide a novel strategy for healing some estradiol-related diseases in clinic. Methods BMMs were obtained from 6-week-old male C57BL/6J mice. BMMs were labeled with 17β-estradiol-BSA-FITC or BSA-FITC, and fluorescence binding was detected by confocal laser scanning microscopy and flow cytometry. Then, BMMs were incubated with 17β-estradiol-BSA-FITC in the presence of 10-fold excess of 17β-estradiol, 17p-estradiol-BSA, tamoxifen or testosterone, and fluorescence intensity was analyzed by flow cytometry. Results Confocal laser scanning microscopy showed that 17β-estradiol-BSA-FITC was able to bind on the plasma membrane of BMMs. Flow cytometry indicated that the binding of 17β-estradiol-BSA-FITC was competitively reduced by 17β-estradiol and 17|3-es-tradiol-BSA, whereas tamoxifen and testosterone had no influence on 17p-estradiol-BSA-FITC binding.Conclusion It is reasonable to assume that there are estradiol receptors on the surface of BMMs.
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维普
万方数据
