Objective To observe the effect of short-term pretreatment with atorvastatin calcium on regulating expression of matrix metalloproteinase-2 ( MMP-2) and tissue inhibitor of metalloproteinase-2 (TIMP-2) in myocardial ischemia reperfusion injury rabbits model. Methods The rabbits were randomly divided into normal control group, placebo group, atorvastatin calcium group. Myocardial ischemia reperfusion injury model was built according to traditional methods. After ischemia 30min, then reperfusion 180min, the area of ischemic and infarcted myocardium was evaluated via TTC staining, the expression level of MMP-2 and TIMP-2 in ischemia/reperfusion myocardium were evaluated via mean optical density. Result the area of ischemic myocardium of atorvastain calcium group (37.5 ±1.50) wasn' t signifcantly different from placebo group (38. 61 ± 1. 53), but the infarcted area (29. 67 ±2. 08) was significantly lower than that of placebo group (50.00±2.00) (P < 0.01). The expression level of MMP-2 in myocardial cytoplasm was signifcantly higher in placebo group (1.17 ±0.09) than normal control group(0.23 ± 0.04) and atorvastatin calcium group (0.21 ±0.03) (all P <0.05). But the expression level of TIMP-2 was signifcantly higher in atorvastatin calcium group (0. 24 ±0.02) than placebo group (0. 17 ±0. 02) ( P <0.05). Conclusion The cardioprotective mechanism of atorvastation calcium on myocardial ischemia reperfusion injury maybe involved in inhibiting the expression of MMP-2 and upregulating the expression of T1MP-2.
atorvastatin calciuischemia reperfusion injurypreconditioningmetalloproteinase 2tissue inhibitor of metalloproteinase 2rabbits
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维普
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