The effect of maternal water deprivation during late gestation on water-electrolyte metabolism related to renin-angiotensin system in the offspring rats
Objective To determine the effect of maternal water deprivation during late gestation on water-electrolyte metabolism related to renin-angiotensin system( RAS) in the offspring rats. Methods Maternal rats were deprivated of water for 3 days during late gestation period, The arterial blood PO2 > PCO2 ,pH ,Na + /K + concentrations andplasma osmotic pressure of 5-month old offspring were measured. Angiotensin receptor proteins expression in the forebrain were determined by western blot, And intake of 1. 8% NaCl and water in the following 2 hours after intracerebroventricular (i. c. v) angiotensin Ⅱ were measured. Results Blood values (PO2 , PCO2 ,pH,Na+/K+ concentrations and osmolality) were not changed by maternal water deprivation during late gestalion . AT, R protein expression in the forebrain was increased significantly in both male and female adult offspring, while AT,R protein expression was increased only in females. Their ratio of AT,/AT2was increased in males, but not females. Ⅰ. c. v angiotensin Ⅱ-induced water intake in male increased significantly, but not female. Conclusions Maternal water deprivation during late gestation could alter water-electrolyte metabolism, which was associated with the changes of the expression of AT, and AT2 receptors in the forebrain of the adult offspring.
dehydration during late gestationadult offspringRASwater-electrolyte metabolism
NETL
NSTL
维普
万方数据
